| Wang Ping,Li Jie,Xuan Yuelan,Han Lin,Wang Xiai,Wang Zhaonan,Lyu Yumin.The feasibility of using automatic micronucleus assay to test micronucleus in lymphocyte of radiation workers[J].Chinese Journal of Radiological Medicine and Protection,2020,40(10):746-752 |
| The feasibility of using automatic micronucleus assay to test micronucleus in lymphocyte of radiation workers |
| Received:March 31, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.10.003 |
| KeyWords:Radiation workers Lymphocyte Micronucleus Automatic analysis |
| FundProject:河南省重点科技攻关项目（142102310086）；河南省医学科技攻关计划项目（LHGJ20190815） |
| Author Name | Affiliation | E-mail | | Wang Ping | Henan Institute of Occupational Medicine, Zhengzhou 450052, China | | | Li Jie | Henan Institute of Occupational Medicine, Zhengzhou 450052, China | | | Xuan Yuelan | Henan Institute of Occupational Medicine, Zhengzhou 450052, China | | | Han Lin | Henan Institute of Occupational Medicine, Zhengzhou 450052, China | | | Wang Xiai | Henan Institute of Occupational Medicine, Zhengzhou 450052, China | | | Wang Zhaonan | Henan Institute of Occupational Medicine, Zhengzhou 450052, China | | | Lyu Yumin | Henan Institute of Occupational Medicine, Zhengzhou 450052, China | lym636160@sina.com |
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| Abstract:: |
| Objective To explore the feasibility of using a High-Throughput Chromosome Automatic Scanning System (Metafer 4) to carry out automatic micronucleus detection for radiation workers. Methods A total of 2 005 radiation workers selected were divided into five groups in terms of diagnostic radiology, radiotherapy, nuclear medicine, interventional radiology and industrial application.The Metafer 4 was used to assay micronuclei in peripheral blood lymphocytes from the subjects, with the factors affecting micronuclei analyzed. A total of 200 micronucleus samples collected by stratified random sampling were manually assayed for comparison of micronuclei frequency and detection efficiency between automatic and manual analysis. Results The mean micronuclei frequency in 2 005 radiation workers were measured to be (0.92±0.02)×10-3. There was significant difference in the frequencies of micronuclei among the five groups (χ2=11.054,P<0.05), with higher micronuclei frequency in interventional radiology group(1.00±0.03)×10-3 than in other groups (χ2=5.568,5.334,7.689,P<0.05 ). Statistically significant difference could be observed in annual effective dose, length of service and micronuclei frequency among the radiation workers in different medical applications (F=3.026, χ2=11.582, 8.878,P<0.05 ). A positive correlation between annual effective dose and micronucleus frequency was shown by Pearson analysis (r=0.106,P<0.05 ). Multiple linear regression analysis showed that the automatic assay-based micronuclei frequencies were associated with length of service and work categories(r=9.485×10-6, 5.660×10-5,P<0.05), significantly lower when compared with manual analysis (χ2=169.259,P<0.05 ). There was a consistency (ICC=0.713,P<0.05) between the two assay methods, but automatic analysis can increase the assayed micronucleus frequency by about 5 folds. Conclusions The micronucleus frequency tested by automatic detection reflects genetic damage from low-dose chronic radiation exposure, and there may be feasibility of using automatic analysis to assay micronucleus of radiation workers. |
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