| Yang Xuejiao,Shi Wenyu,Ma Jiayan,Ma Linlin,Li Mengting,Ye Zhujing,Cao Jianping,Zhang Liyuan,Yang Hongying.Irradiated glioma cells induce bystander effects in neural stem cells[J].Chinese Journal of Radiological Medicine and Protection,2020,40(9):659-665 |
| Irradiated glioma cells induce bystander effects in neural stem cells |
| Received:January 07, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.09.002 |
| KeyWords:Ionizing radiation Bystander effects Neural stem cells Glioma cells |
| FundProject:国家自然科学基金（U1632270，81773362） |
| Author Name | Affiliation | E-mail | | Yang Xuejiao | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, State Key Laboratory of Radiation Medicine and Radiation Protection, Suzhou 215123, China | | | Shi Wenyu | Department of Radiotherapy&Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Ma Jiayan | Department of Radiotherapy&Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Ma Linlin | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, State Key Laboratory of Radiation Medicine and Radiation Protection, Suzhou 215123, China | | | Li Mengting | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, State Key Laboratory of Radiation Medicine and Radiation Protection, Suzhou 215123, China | | | Ye Zhujing | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, State Key Laboratory of Radiation Medicine and Radiation Protection, Suzhou 215123, China | | | Cao Jianping | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, State Key Laboratory of Radiation Medicine and Radiation Protection, Suzhou 215123, China | | | Zhang Liyuan | Department of Radiotherapy&Oncology, Second Affiliated Hospital of Soochow University, Suzhou 215004, China | | | Yang Hongying | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, State Key Laboratory of Radiation Medicine and Radiation Protection, Suzhou 215123, China | yanghongying@suda.edu.cn |
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| Abstract:: |
| Objective To investigate whether irradiated U251 glioma cells can induce bystander effects in unexposed neural stem cells (NSCs) thus affecting its proliferation, stemness and differentiation. Methods The cells were divided into NSCs group, NSCs+U251 group (co-cultured with U251) and NSCs+ IR U251 group (co-cultured with 10 Gy irradiated U251). Glioma cells and NSCs were co-cultured in a transwell insert set. Cell counting and neurosphere diameter measuring were carried out to evaluate the proliferation and neurosphere formation ability of NSCs. Immunofluorescence assay was performed to detect the expression of Nestin protein to evaluate the stemness maintenance of NSCs, and to measure the expression levels of Tuj1 and GFAP proteins, the number of neuronal dendrites, synaptic length, the number of glial protrusions, as well as the length of glial protrusions. Results The number of NSCs cultured with irradiated U251 cells was obviously smaller than that of NSCs cultured with sham-irradiated U251 cells (t=2.52, P<0.05). The neurosphere formation ability of NSCs and the percentage of Nestin positive NSCs after co-culture with irradiated U251 cells significantly reduced in comparison with those after co-culture with sham-irradiated U251 cells (t=-3.50, P<0.05). The percentages and the extent of NSCs differentiating into neuronal cells and glial cells(t=6.09, P<0.05)decreased obviously after co-culture with irradiated U251 cells in comparison with those after co-culture with sham-irradiated U251 cells. Conclusions Irradiated glioma cells can significantly inhibit the proliferation, stemness and differentiation of unexposed NSCs due to bystander effect. |
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