| Ji Jianfeng,Liu Yunying,Kong Yue,et al.Experimental study in the evaluation of radiation-induced cognitive dysfunction in rats via 18F-FDG micro-PET metabolic imaging[J].Chinese Journal of Radiological Medicine and Protection,2020,40(9):653-658 |
| Experimental study in the evaluation of radiation-induced cognitive dysfunction in rats via 18F-FDG micro-PET metabolic imaging |
| Received:February 21, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.09.001 |
| KeyWords:18F-FDG micro-PET Whole-brain irradiation Cognitive dysfunction Hippocampus c-Fos |
| FundProject:国家自然科学基金（81602672）；浙江省医药卫生科研项目（2019324448，2019327308） |
| Author Name | Affiliation | E-mail | | Ji Jianfeng | Department of Nuclear Medicine, Cancer Hospital of University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou 310022, China | | | Liu Yunying | Department of Pathology, Cancer Hospital of University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou 310022, China | | | Kong Yue | Department of Radiation Oncology, Cancer Hospital of University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou 310022, China | | | Gu Qing | Department of Radiation Oncology, Cancer Hospital of University of Chinese Academy of Sciences, Zhejiang Cancer Hospital, Hangzhou 310022, China | guqing@zjcc.org.cn |
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| Abstract:: |
| Objective To investigate the correlation between glucose metabolism and neuron activity in radiation-induced brain injury of rat, and to explore the potential implication of 18F-FDG micro-PET in the assessment of radiation-induced cognitive dysfunction. Methods Three-week-old male Sprague-Dawley (SD) rats were divided into two groups, whole-brain irradiation (WBI) group and non-irradiation control group, according to the random number table method. The WBI group was irradiated with 10 Gy X-rays using a small animal precise radiotherapy apparatus. Morris water maze (MWM) test was performed to evaluate the cognitive capability of rats. 18F-FDG micro-PET covering the whole brain was conducted and the micro-PET images were processed by SPM software. The expression of neuronal activity marker c-Fos protein in rat brain was detected by immunohistochemical staining. The neuronal precursors marker DCX positive cells and newborn mature neurons marker BrdU/NeuN positive cells were detected by immunofluorescence staining. Results Three months after irradiation, MWM place navigation test showed that the latency of whole-brain irradiated rats was longer than that of the control group (t=2.179, 3.393, 3.219, P<0.05). In MWM spatial probe test, the percentage of target quadrant exploring time was reduced in the WBI group compared with the control group (t=3.857, P<0.01). These result suggested that WBI caused hippocampus injury-related cognitive decline. SPM analysis of micro-PET images showed that, after WBI, the glucose metabolism in the hippocampus was significantly reduced (t=5.12, P<0.05), the neuronal active marker c-Fos protein expression was significantly downregulated (t=14.22, P<0.01), and the neuronal precursors marker DCX positive cells and newborn mature neurons marker BrdU/NeuN positive cells were both decreased (t=18.77, 9.304, P<0.01). Conclusions Glucose metabolism in the hippocampus was reduced after WBI, in consistent with the decrease of neuron activity and the reduction of neurogenesis in this area, suggesting that 18F-FDG micro-PET could be an effective method for assessing radiation-induced cognitive dysfunction. |
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