| Yin Jun,Li Xinyue,Zhang Xuxia,Wang Mengmeng,Chen Yu,Chen Honghong.Radiosensitzation effect of Ta4C3-PVP nanosheets in human triple-negative breast cancer MDA-MB-231 cells[J].Chinese Journal of Radiological Medicine and Protection,2020,40(7):493-499 |
| Radiosensitzation effect of Ta4C3-PVP nanosheets in human triple-negative breast cancer MDA-MB-231 cells |
| Received:January 16, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.07.001 |
| KeyWords:Tantalum carbide Nanosheets Radiosensitization Triple-negative breast cancer |
| FundProject:国家自然科学基金（31300694，81673091） |
| Author Name | Affiliation | E-mail | | Yin Jun | Institute of Radiation Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China | | | Li Xinyue | Institute of Radiation Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China | | | Zhang Xuxia | Institute of Radiation Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China | | | Wang Mengmeng | Institute of Radiation Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China | | | Chen Yu | Shanghai Institute of Ceremics Chinese Academy of Sciences, Shanghai 200050, China | | | Chen Honghong | Institute of Radiation Medicine, Shanghai Medical College, Fudan University, Shanghai 200032, China | hhchen@shmu.edu.cn |
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| Abstract:: |
| Objective To assess the radiosensitivity of polyvinylpyrrolidone (PVP) modified tantalum carbide (Ta4C3) nanosheets in human triple-negative breast cancer MDA-MB-231 cells in vitro. Methods PVP-modified Ta4C3 nanosheets were synthesized and characterized. The uptake of fluoresceine isothiocyanate(FITC) labeled Ta4C3-PVP nanosheets in MDA-MB-231 cells was examined by fluorescent microscope. The toxicity of Ta4C3-PVP in the cells was measured by CCK-8 assay.These cells were divided into 4 groups:control group, Ta4C3-PVP group, irradiation group alone and Ta4C3-PVP plus irradiation group. The colony formation assay was used to evaluate the radiosensitivity. The levels of intracellular reactive oxygen species (ROS) and lipid oxidation production, malondialdehyde (MDA), were detected with an automatic microplate reader.γH2AX foci and mitotic catastrophe were observed by immunofluorescence staining. Results Ta4C3-PVP nanosheets with a single-layer flaky shape were successfully synthesized. The hydrodynamic diameter and thick of Ta4C3-PVP nanosheets were about 143.93 nm and 1.35 nm, respectively. The FITC labeled Ta4C3-PVP nanosheets were uptaken by MDA-MB-231 cells, which was mainly distributed in the cytoplasm.Treatment of Ta4C3-PVP nanosheets at concentrations up to 400 μg/ml was noncytotoxic to MDA-MB-231 cells. The colony formation assay showed that pretreatment with Ta4C3-PVP at 50 and 100 μg/ml moved the survival curve of the irradiated cells downward in a concentration-dependent manner, compared to irradiation group alone. The sensitization enhancement ratio (SERD0) of Ta4C3-PVP at 50 and 100 μg/ml were 1.21 and 1.45, respectively.The intracellular ROS level in Ta4C3-PVP plus irradiation group was significantly higher than that in irradiation group alone at 2 and 12 h after irradiation (q=20.01, 7.193, P<0.05). The percentage of positive cells with more than five γH2AX foci in Ta4C3-PVP plus irradiation group was higher than that of irradiation group alone at 1, 4 and 8 h after irradiation (q=36.78, 14.87, 8.217, P<0.05). The content of MDA in Ta4C3-PVP plus irradiation group was significantly higher than that of irradiation group alone at 24 and 48 h after irradiation (q=14.02, 7.015, P<0.05). The percentage of cells that succumbed to mitotic catastrophe in Ta4C3-PVP plus irradiation group was significantly higher than that in irradiation group alone at 72 h after irradiation (q=16.33, P<0.05). Conclusions Ta4C3-PVP nanosheets could enhance the radiosensitivity of human triple-negative breast cancer MDA-MB-231 cells through an increase of the intracellular ROS induced by irradiation. |
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