| Yue Xiaoqiao,Bai Chenjun,Xie Dafei,Liu Xiaodan,Zhou Pingkun.Identification and targets prediction of snoRNAs in α-particle induced carcinogenesis of human branchial epithelial cells[J].Chinese Journal of Radiological Medicine and Protection,2020,40(6):413-419 |
| Identification and targets prediction of snoRNAs in α-particle induced carcinogenesis of human branchial epithelial cells |
| Received:January 28, 2020 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.06.001 |
| KeyWords:α-particles Ionizing radiation snoRNA sno116-14 Cancerous |
| FundProject:国家自然科学基金（81530085，11705283）；湖南省研究生科研创新项目（CX20190779） |
| Author Name | Affiliation | E-mail | | Yue Xiaoqiao | The School of Public Health, University of South China, Hengyang 421001, China | | | Bai Chenjun | The Institute of Radiation Medicine, The Academy of Military Medical Science, Beijing 100850, China | | | Xie Dafei | The Institute of Radiation Medicine, The Academy of Military Medical Science, Beijing 100850, China | | | Liu Xiaodan | The Institute of Radiation Medicine, The Academy of Military Medical Science, Beijing 100850, China | | | Zhou Pingkun | The School of Public Health, University of South China, Hengyang 421001, China | zhoupk@bmi.ac.cn |
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| Abstract:: |
| Objective To identify the differentially expressed snoRNAs in the carcinogenesis of cells induced by α-particles radiation and predict the targeted genes and RNA-co-expression networks. Methods Full transcriptome expression microarray biochips were employed to screen the differentially expressed snoRNAs between human bronchial epithelial BEP2D cell line and its derivative malignantly transformed cell line BERP35T-4 established by α-particle irradiation. The expression changes of snoRNAs and their derived sdRNAs were confirmed by qRT-PCR. The functional domains, targets and co-expression networks of snoRNA were predicted by bioinformatics analysis. Results Consistent with the result of microarray assay, the expression changes of the screened snoRNAs were confirmed by qRT-PCR. The expressions of sno116 family decreased in BERP35T-4, which was 0.105% (t=26.60, P<0.01) of BEP2D, and they were generally down-regulated in radiation-induced carcinogenic BERP35T-4 cells and the human lung cancer cell lines A549 and H1299. It was also found that the expression level of the sdRNAs derived from sno116-14 was significantly different in the same cells. It was speculated that these less expressed sdRNAs of sno116-14 could be due to degradation as the consequence of interaction with their targets. The co-expression networks of sno116 family with other types of RNA were established, and the predicted targets of sno116-14 included ZNF280D, TFDP1, CCDC28B, RPS6KA3, CANX, RUNX1 and KALRN, which were related to the functions of cell proliferation and cytoskeletal structure. Conclusions Some differentially expressed snoRNAs related to α-particle induced carcinogenesis have been identified. It is predicted that the target gene of sno116-14 is involved in the biological processes such as cell proliferation, cytoskeletal structure and the signaling pathways for function regulation, providing new information for the function model of C/D box snoRNAs and the mechanism of radiation carcinogenesis. |
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