| Kuang Huaxiang,Xu Shilin,Ouyang Weiwei,Zhao Chaofen,Li Xiaoyang,Chen Xiaxia,Yang Wengang,Ma Zhu,Lu Bing,Su Shengfa.Recombinant human endostatin reduces radiation-induced myocardial fibrosis in rats[J].Chinese Journal of Radiological Medicine and Protection,2020,40(5):343-348 |
| Recombinant human endostatin reduces radiation-induced myocardial fibrosis in rats |
| Received:September 13, 2019 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.05.003 |
| KeyWords:Recombinant human endostatin Radiation Heart Fibrosis |
| FundProject:国家自然科学基金（81660507）；贵州省科技支撑计划项目（[2018]2755） |
| Author Name | Affiliation | E-mail | | Kuang Huaxiang | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | | | Xu Shilin | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | | | Ouyang Weiwei | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | | | Zhao Chaofen | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | | | Li Xiaoyang | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | | | Chen Xiaxia | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | | | Yang Wengang | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | | | Ma Zhu | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | | | Lu Bing | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | | | Su Shengfa | Department of Oncology, Affiliated Hospital of Guizhou Medical University, Teaching and Research Section of Oncology, Guizhou Medical University, Department of Oncology, Guizhou Cancer Hospital, Guiyang 550004, China | sushengfa2005@163.com |
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| Abstract:: |
| Objective To assess the effects of recombinant human endostatin (rh-ES) on radiation-induced myocardial fibrosis. Methods Totally 40 SD rats were randomly divided into 4 groups, including A group as normal control, B group receiving rh-ES with a dosage of 6 mg·kg-1·d-1, in traperitoneal injection, for 14 consecutive days, C group with local heart irradiation delivered to the precordial region of rats in five fractions with a dose of 25 Gy, D group receiving rh-ES as the same as B group and local heart irradiation as C group. At 1 and 3 months after irradiation, five rats were killed under anesthesia. Mason staining was used to observe myocardial injury and fibrosis. Western blotting was used to detect the expression of TGF-β1, CTGF and COL-I in myocardium. Results Masson staining showed that no obvious myocardial fibrosis was found in group B at 1 month and 3 months after irradiation, while collagen fibers were distributed in myocardium in groups C and D. One month after irradiation, the result of semi-quantitative analysis showed that the CVF in group A was (5.20 ±0.75)%, which was significantly lower than that in group C (10.12 ±2.17)% (t=4.74、4.93,P<0.01) and the CVF in group D (10.32 ±1.36),and the CVF of group C was similar to that of group D (P<0.01). Three months after irradiation, CVF in group C (13.17±2.67)% was still higher than that in group A (5.23 ±1.32)% (t=4.49, P<0.01), but lower than that in group D (16.92 ±3.58)% (t=3.19,P<0.05). One month after irradiation, the expression of TGF-β1 in group A was 0.441 ±0.063, lower than that in group C (0.817 ±0.079, t=5.81, P<0.01). Three months after irradiation, the expression of TGF-β1 in group A was 0.501 ±0.110, lower than that in group C (0.832 ±0.150, t=4.19,P<0.01), and the expression of TGF-β1 in group D was 1.403 ±0.133, which was significantly higher than that in group C (t=7.24, P<0.01). Conclusions Radiation can cause the formation of myocardial fibrosis, and recombinant human endostatin may aggravate the formation of late radiation fibrosis. |
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