| Yan Juan,Liu Qingjie,Tian Mei,et al.Premature senescence and the protein expressions of P53 and P16 in HaCaT cells after UVB exposure[J].Chinese Journal of Radiological Medicine and Protection,2020,40(4):284-289 |
| Premature senescence and the protein expressions of P53 and P16 in HaCaT cells after UVB exposure |
| Received:October 16, 2019 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.04.007 |
| KeyWords:Ultraviolet B Premature senescence Keratinocytes P53 P16 |
| FundProject:北京市自然科学基金（7162137，7202139） |
| Author Name | Affiliation | E-mail | | Yan Juan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Qingjie | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Mei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Lu Xue | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Cai Tianjing | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Li Shuang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Zhao Hua | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Xuelei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Chen Deqing | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Gao Ling | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | gaolingxinxin@126.com |
|
| Hits: 4042 |
| Download times: 1592 |
| Abstract:: |
| Objective To explore the effects of ultraviolet B (UVB) on the premature senescence of human immortalized keratinocytes HaCaT cells and the possible underlying molecular mechanism. Methods HaCaT cells were exposed with UVB of different doses (20, 50, 80 and 100 mJ/cm2). At 72 h after exposure, cellular morphology was observed by Giemsa staining, cell proliferation was detected by clone formation assay, and the proportion of premature senescence cells was detected by β-galactosidase staining. The number change of lysosomes was detected by Lyso-Tracker Red fluorescence probe at 24, 48 and 72 h after exposure. Cell migration was measured by scratch test at 24 h and 48 h after exposure. The protein expressions of p53 and p16 related to premature senescence were detected by Western blot assay at 72 h after exposure. Results After UVB exposure, HaCaT cells showed a premature senescence phenotype. At 72 h after exposure, the cell volume increased (F=115.18,P<0.05), the cell proliferation ability decreased (F=410.32, P<0.05), the activity of β-galactosidase increased (F=16.31,P<0.05), and the expressions of P53 and P16 increased. In addition, the number of lysosomes increased at 24, 48, and 72 h after exposure (F=17.65, 38.36, 13.66, P<0.05),and cell migration capacity was inhibited at 24 and 48 h after exposure (F=8.21, 11.48, P<0.05). Conclusions UVB exposure can induce premature senescence of HaCaT cells by increasing the expression of p53 and p16 proteins. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
