| Yao Dan,Zhang Junling,Zheng Wang,et al.Effect of fractionated radiotherapy of transplanted hepatocellular carcinoma on the splenic immune cells in mice[J].Chinese Journal of Radiological Medicine and Protection,2020,40(2):82-87 |
| Effect of fractionated radiotherapy of transplanted hepatocellular carcinoma on the splenic immune cells in mice |
| Received:August 29, 2019 |
| DOI：10.3760/cma.j.issn.0254-5098.2020.02.002 |
| KeyWords:Hepatocellular carcinoma Fractionated radiotherapy Splenic lymphocytes |
| FundProject:国家重点研发计划项目（2017YFC0108604）；国家自然科学基金（31770910，31570850） |
| Author Name | Affiliation | E-mail | | Yao Dan | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Zhang Junling | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Zheng Wang | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Chen Qianping | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Hu Songling | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Pan Yan | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Shao Chunlin | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | clshao@shmu.edu.cn |
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| Abstract:: |
| Objective To investigate the effect of fractionated radiotherapy on the immune system of mice with subcutaneously transplanted hepatocellular carcinoma. Methods Logarithmic growth of mouse hepatocellular carcinoma cells Hepa 1-6 were inoculated subcutaneously on the right side of C57BL/6 J mice (1×107 cells/mice). The tumor-bearing mice were randomly divided into control group (Ctrl) and irradiation group (IR), 20 mice in each group. Additionally, 10 healthy mice were set as normal control group. Local fractionated X-ray irradiation of 8 Gy×3 fraction was given to the subcutaneous tumors, and the dose rate was 0.883 Gy/min. At 7 and 14 d after irradiation, the tumor organ index, spleen organ index, spleen pathological changes, and splenic T lymphocyte subsets, B lymphocyte subsets, and NK cells were detected. Results Compared with Ctrl, at 7 and 14 d after irradiation, the tumor organ index decreased (t=4.649, 26.34,P<0.05), and the percentage of NK cells increased significantly (t=3.952, 3.633,P<0.05). The percentages of CD3+, CD4+, CD3+CD4+ lymphocytes and the ratio of CD4+/CD8+ lymphocyte decreased at 7 d after irradiation (t=3.193, 3.656, 3.219, 2.641,P<0.05), and the percentage of CD3+lymphocyte decreased at 14 d after irradiation (t=3.031,P<0.05). But after irradiation, there were no significant changes in spleen organ index, B lymphocyte, CD3+ CD4+ lymphocyte and CD8+ lymphocyte. Conclusions Local hepatoma radiotherapy causes imbalance of lymphocytes in distal spleen of mice and hence reduces immunity, which provides a novel mechanism of radiological immunity damage. |
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