| Sun Xiantao,Lian Yanbang,Bai Yang,et al.Effect of lncRNA CRNDE targeting miR-384 on radiosensitivity of colorectal cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2019,39(12):893-898 |
| Effect of lncRNA CRNDE targeting miR-384 on radiosensitivity of colorectal cancer cells |
| Received:March 04, 2019 |
| DOI：10.3760/cma.j.issn.0254-5098.2019.12.003 |
| KeyWords:Colorectal cancer Colorectal neoplasia differentially expressed(CRNDE) miR-384 Radiosensitivity |
| FundProject: |
| Author Name | Affiliation | E-mail | | Sun Xiantao | Department of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Lian Yanbang | Department of Radiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Bai Yang | Department of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Yang Chao | Department of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Hu Shengyun | Department of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Wang Guixian | Department of Colorectal Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | sz90201459@sina.com |
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| Abstract:: |
| Objective To study the effect of LncRNA CRNDE on radiosensitivity of colorectal cells and underlying mechanism. Methods Colorectal cancer HT-29 cells were transfected with CRNDE shRNA and the interference efficiency was determined by Real time PCR. HT-29 cells transfected with CRNDE shRNA or co-transfected with CRNDE shRNA and miR-384 inhibitor were irradiated at 8 Gy dose, then cell proliferation and apoptosis were detected by MTT and flow cytometry assay, respectively, and cell radiosensitivity was evaluated by cloning assay. It was predicted by a bioinformatics software that CRNDE and miR-384 have complementary binding sites, and this was identified by a luciferase reporting system. Results CRNDE shRNA reduced the expression of CRNDE in HT-29 cells(1.00±0.08 vs. 0.42±0.06, t=10.051, P<0.05). Both CRNDE shRNA and radiation inhibited the proliferation and induced apoptosis of HT-29 cells, and their combination treatment had synergistic effect in apoptosis induction[Apoptosis rates:(2.27±0.13)%, (23.58±2.35)%, (26.91±2.81)%, (36.84±3.24)%,F=24.660,P<0.05;A values:0.45±0.06, 0.30±0.02, 0.28±0.03, 0.20±0.02, F=106.207, P<0.05]. Transfection of CRNDE shRNA increased the radiosensitivity of HT-29 cells with a radiosensitization ratio of 1.374. CRNDE negatively regulated the expression of its target miR-384. The miR-384 inhibitor antagonized the effect of CRNDE shRNA on proliferation inhibition and apoptosis promotion of radiation-treated colorectal cancer cells. Conclusions Down-regulation of LncRNA expression enhances the radiosensitivity of colorectal cancer cells by regulating miR-384 expression. |
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