| Xu Guangmin,Yang Yaning,Xu Shengmin,Wu Lijun.The role of ceramide in radiation-induced bystander effect on germ cells in Caenorhabditis elegans[J].Chinese Journal of Radiological Medicine and Protection,2019,39(6):408-414 |
| The role of ceramide in radiation-induced bystander effect on germ cells in Caenorhabditis elegans |
| Received:January 26, 2019 |
| DOI：10.3760/cma.j.issn.0254-5098.2019.06.002 |
| KeyWords:Radiation induced bystander effect(RIBE) Ceramide Caenorhabditis elegans Germ cell Apoptosis |
| FundProject:国家自然科学基金（31470829） |
| Author Name | Affiliation | E-mail | | Xu Guangmin | Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China | | | Yang Yaning | University of Science and Technology of China, Hefei 230026, China | | | Xu Shengmin | Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei 230031, China | | | Wu Lijun | Institutes of Physical Science and Information Technology, Anhui University, Hefei 230601, China | ljw@ipp.ac.cn |
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| Abstract:: |
| Objective To investigate the role of ceramide in radiation induced bystander effect (RIBE) in vivo by irradiating Caenorhabditis elegans with proton microbeam. Methods The posterior pharynx of wide-type N2 and genetically mutated L4-staged worms was irradiated with 2 000 particles, then germ cell apoptosis and gene expression were analyzed. Results Point-fixed radiation to posterior pharynx of N2 worms significantly increased the number of apoptotic germ cells (t=9.007, P<0.05), but not in the worms with deletion mutants of ceramide synthase (CerS) genes (hyl-1 and lagr-1) (P>0.05). Real-time quantitative PCR assay indicated that in both N2 and lagr-1(gk327);hyl-1(ok976) worms, the genes of egl-1 and ced-13 in core apoptosis pathway were up-regulated after radiation to posterior pharynx, without statistical significance between these two strains (P>0.05). The gene expression levels of hyl-1 and lagr-1 were increased in both N2 and hus-1(op241) worms after radiation with no statistical significant difference between two strains (P>0.05). Furthermore, radiation-induced germ cell apoptosis was increased in abl-1(ok171) worms (t=13.241, P<0.05), rather than in lagr-1(gk327);hyl-1(ok976);abl-1(ok171) worms (t=13.462, P<0.05). Conclusions Ceramide is required for RIBE on germ cell apoptosis in C. elegans, and it might play function together with egl-1 and ced-13 in core apoptosis pathway and HUS-1 in DNA damage response pathway. But ceramide had antagonistic relationship with anti-apoptotic protein abl-1 in germ cell apoptosis. |
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