| Chen Qin,Gao Peng,Hu Junfeng,Ding Guirong,Guo Guozhen.Effect of low-frequency pulsed magnetic field on blood-brain barrier permeability of rat hippocampus[J].Chinese Journal of Radiological Medicine and Protection,2019,39(6):401-407 |
| Effect of low-frequency pulsed magnetic field on blood-brain barrier permeability of rat hippocampus |
| Received:March 26, 2019 |
| DOI：10.3760/cma.j.issn.0254-5098.2019.06.001 |
| KeyWords:Pulsed magnetic field Rats Hippocampus Blood brain barrier Tight junction protein |
| FundProject:国家自然科学基金重点项目（51437008）；国家基础加强计划重点基础研究项目（2017-JCJQ-ZD-051） |
| Author Name | Affiliation | E-mail | | Chen Qin | Department of Radiation Protection Medicine, Air Force Medical University, Xi'an 710032, China | | | Gao Peng | Department of Radiation Protection Medicine, Air Force Medical University, Xi'an 710032, China | | | Hu Junfeng | Department of Radiation Protection Medicine, Air Force Medical University, Xi'an 710032, China | | | Ding Guirong | Department of Radiation Protection Medicine, Air Force Medical University, Xi'an 710032, China | | | Guo Guozhen | Department of Radiation Protection Medicine, Air Force Medical University, Xi'an 710032, China | guozhen@fmmu.edu.cn |
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| Abstract:: |
| Objective To investigate the effect of pulsed magnetic radiation on BBB permeability of hippocampus in Sprague-Dawley rats at different exposure intensity. Methods A total of 72 male SD rats were randomly divided into sham exposure group, positive control group and magnetic field treatment groups (100, 400, 800, 1 200 mT, 30 pulses each group). At three hours after exposure, the morphological structure of hippocampus was evaluated by HE staining, the extravasation of albumin around microvessels in rat hippocampus was detected immunohistochemically, the EB extravasation around microvessels was observed with Evans blue (EB) fluorescence method, and the levels of BBB-related protein ZO-1 and Occludin in hippocampus were measured with Western blot assay. Results Compared with the control group, no significant change in the hippocampus morphology structure, the extravasation of albumin and EB around the microvessels were observed after the pulsed magnetic exposures. The protein levels of ZO-1 and Occludin had no changes in the exposed groups(P>0.05) except that ZO-1 was significantly reduced in 1 200 mT exposure group(t=14.26,P<0.05). Conclusions Low-frequency pulsed magnetic field could not affect the permeability of BBB in SD rats but impairs the integrity of BBB at 1 200 mT. |
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