| Ma Jian,Wang Xinjun,Fu Xudong,et al.miR-124 increases the radiosensitivity of glioblastoma cells through targeting STAT3[J].Chinese Journal of Radiological Medicine and Protection,2019,39(2):88-94 |
| miR-124 increases the radiosensitivity of glioblastoma cells through targeting STAT3 |
| Received:July 24, 2018 |
| DOI：10.3760/cma.j.issn.0254-5098.2019.02.002 |
| KeyWords:Glioma cells Radiosensitivity miR-124 STAT3 |
| FundProject: |
| Author Name | Affiliation | E-mail | | Ma Jian | Department of Neurosurgery, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China | | | Wang Xinjun | Department of Neurosurgery, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China | wangxj@zzu.edu.cn | | Fu Xudong | Department of Neurosurgery, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China | | | Zhou Shaolong | Department of Neurosurgery, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China | | | Tian Bi | Department of Radiotherapy, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China | | | Yan Lin | Department of Oncology, The Fifth Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China | |
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| Abstract:: |
| Objective To investigate the expression of miR-124 in glioblastoma (GBM) cell lines LN229 and LN229R, as well as the regulatory mechanism of miR-124 on radiosensitivity of LN229R cells. Methods miR-124 mimic (miR-124) and negative control (miR-NC), STAT3 overexpression plasmid (STAT3) and pcDNA3.1 vector (pcDNA) were transfected or co-transfected into radioresistant glioma cells LN229R. qRT-PCR was employed to analyze the expression of miR-124 in LN229 and LN229R cells. The survival rate and sensitivity-related parameters of LN229R cells at different doses were analyzed by cloning formation assay. Cell apoptosis of LN229R was evaluated by flow cytometry. Targeting gene of miR-124 was predicted using Targetscan software and verified by the double-luciferase reporter assay. Western blot assay was performed to detect STAT3 protein expression. Results The expression of miR-124 in LN229R cells (0.32±0.03) was significantly lower than that in LN229 cells (1.02±0.09) (t=12.780, P<0.05). Transfection of miR-124 mimics promoted the expression of miR-124 in LN229R cells (4.02±0.39) compared with miR-NC group (0.95±0.06) (t=13.476, P<0.05). After 8 Gy irradiation, the survival rate of LN229R cells transfected with miR-124 mimics (0.003±0.000 4) was significantly lower than that in miR-NC group (0.033±0.005 0) (t=5.655, P<0.05), and the apoptosis rate (22.34±2.42)% was significantly higher than that in miR-NC group (4.69±0.51)% (t=12.361, P<0.05). STAT3 was identified to be a target gene of miR-124. Exogenous restoration of STAT3 reversed the inhibitory effect of miR-124 on LN229R cell survival. Conclusion miR-124 increases the radiosensitivity of LN229R cells by targeting STAT3. |
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