| Liu Yang,Jiang Yue,Mao Ronghu,Lu Lingcong,Yang Yuanyuan.Effect of PinX1 on radiosensitivity and ROS level of esophageal cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2019,39(1):37-43 |
| Effect of PinX1 on radiosensitivity and ROS level of esophageal cancer cells |
| Received:May 26, 2018 |
| DOI：10.3760/cma.j.issn.0254-5098.2019.01.008 |
| KeyWords:Esophageal cancer PinX1 Radiation Apoptosis |
| FundProject:河南省科技厅重点科技攻关项目（142102310502）；河南省卫生厅普通科技攻关项目（201403026）；河南省卫生厅科技攻关指导性计划（201304058） |
| Author Name | Affiliation | E-mail | | Liu Yang | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | | | Jiang Yue | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | sunny5799@126.com | | Mao Ronghu | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | | | Lu Lingcong | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | | | Yang Yuanyuan | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou 450008, China | |
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| Abstract:: |
| Objective To study the effect of PinX1 on radiosensitivity and ROS level of esophageal cancer cells.Methods Esophageal cancer cells were divided into control group(no cell transfection), radiation group (8 Gy of X-rays), PinX1 group (pDsRed1-PinX1 transfect to overexpression, radiation+PinX1 group (8 Gy X-rays after pDsRed1-PinX1 transfection). Cell proliferation was measured by MTT assay, cell apoptosis was measured by flow cytometry, the activation levels of Caspase-3 and Caspase-9 in cells were measured by Western blot, the cellular ROS level was determined using DCFH-DA probe, and the cell radiosensitivity was evaluated by the colony formation assay. The subcutaneous xenograft tumor model of EC9706 cells was established with nude mice, and each group received local irradiation every 4 days 8 Gy five fractions γ-rays. The nude mice bearing PinX1-transfected cells were implanted with PinX1 plasmid into tumor before radiotherapy to investigate the relationship between PinX1 overexpression and radiosensitivity in xenotransplantation mice models.Results Compared with control group cells, the survival rate of EC9706 cells of radiation group or PinX1 group decreased significantly from 100% to (67.92±4.71)% and (83.14 ±4.01)% (t=9.433, 4.957, P<0.05), the apoptosis rate increased significantly from(6.47±1.46)% to (44.38±4.16)% and (25.42±2.78)%(t=12.882, 6.439, P<0.05),Caspase-3 activation level increased (t=6.655, 2.531, P<0.05), and the activation level of Caspase-9 and cellular ROS was also significantly increased. Compared with radiation group cells, the cell viability of radiation+PinX1 group decreased from (67.92 ±4.71)% to (52.73 ±5.58)% (t=8.942, P<0.05), the apoptosis rate increased from (44.38±4.16)% to (55.29±4.98)% (t=3.707, P<0.05), the activation levels of Caspase-3,-9, and the level of ROS in cells was increased (t=15.435, 17.990, 4.526, P<0.05). The radiosensitivity of EC9706 cells was increased after PinX1 transfection with a sensitization ratio of 1.408. The volume of xenograft tumor with PinX1 overexpression cells in nude mice was significantly smaller than control.Conclusions Overexpression of PinX1 could inhibit the proliferation, induce apoptosis and increase the radiosensitivity of esophageal cancer cells. |
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