| Li Quanying,Wu Dapeng,Gu Hao,He Zhikuan,Wang Yang,Ge Zheng,Qin Changjiang,Wang Wei.miR-885-3p regulates radiosensitivity of colorectal cancer cell HT-29 by targeting AKT1[J].Chinese Journal of Radiological Medicine and Protection,2018,38(12):899-906 |
| miR-885-3p regulates radiosensitivity of colorectal cancer cell HT-29 by targeting AKT1 |
| Received:June 28, 2018 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.12.004 |
| KeyWords:Colorectal cancer miR-885-3p Radiosensitivity AKT1 |
| FundProject:河南省医学科技攻关计划项目（201601029） |
| Author Name | Affiliation | | Li Quanying | Department of General surgery, Huaihe Hospital of Henan University, Kaifeng 475000, China | | Wu Dapeng | Department of Radiotherapy, Huaihe Hospital of Henan University, Kaifeng 475000, China | | Gu Hao | Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450000, China | | He Zhikuan | Department of General surgery, Huaihe Hospital of Henan University, Kaifeng 475000, China | | Wang Yang | Department of General surgery, Huaihe Hospital of Henan University, Kaifeng 475000, China | | Ge Zheng | Department of General surgery, Huaihe Hospital of Henan University, Kaifeng 475000, China | | Qin Changjiang | Department of General surgery, Huaihe Hospital of Henan University, Kaifeng 475000, China | | Wang Wei | Department of Radiotherapy, Henan Provincial People's Hospital, Zhengzhou 450000, China |
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| Abstract:: |
| Objective To investigate the effect and mechanism of miR-885-3p on the radiosensitivity of colorectal cancer cell HT-29.Methods The expression of miR-885-3p in HT-29 cells irradiated with different doses (0, 2, 4, 6, 8 Gy) of X-rays was detected by qPCR. The effect of miR-885-3p in modulating cell radiosensitivity was assessed in HT-29 cells with miR-885-3p overexpression. Bioinformatics prediction and dual luciferase reporter gene assay were employed to identify the direct target gene of miR-885-3p. Relationship between miR-885-3p and target gene tyrosine kinase 1 (AKT1) was investigated via regulation of miR-885-3p expression. The effect of AKT1 on radiosensitivity in HT-29 cells was evaluated through knockdown AKT1. The effect of AKT1 on miR-885-3p-induced radiosensitivity was detected by co-transferring miR-885-3p and AKT1 gene into HT-29 cells.Results miR-885-3p expression was up-regulated in radiation-induced HT-29 cells (F=46.64, P<0.05). Over-expression of miR-885-3p and knockdown of AKT1 enhanced cell radiosensitization by inhibiting survival and promoting apoptosis (t=12.33, 12.95, P<0.05) with SER of 1.602 and 1.946, respectively. Inhibition of miR-885-3p promoted radioresistance by increasing cell survival and inhibiting apoptosis (t=11.94, P<0.05) with a SER of 0.839. AKT1 is a target gene downstream of miR-885-3p, overexpression of AKT1 reversed the effect of miR-885-3p on cell radiosensitivity with a SER of 0.680.Conclusions miR-885-3p can enhance the radiosensitivity of colorectal cancer HT-29 cells by directly targeting AKT1, which provides a target for improving the radiosensitivity of clinical colorectal cancer. |
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