| Shang Jun,Xie Ting,Yin Narui,et al.Titanium dioxide nanoparticles coupled with nuclear localization sequence increases the radiosensitivity of U251 glioma cells[J].Chinese Journal of Radiological Medicine and Protection,2018,38(11):801-806 |
| Titanium dioxide nanoparticles coupled with nuclear localization sequence increases the radiosensitivity of U251 glioma cells |
| Received:June 01, 2018 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.11.001 |
| KeyWords:Titanium dioxide nanoparticles Nuclear localization sequence Radiosensitivity Glioma cells |
| FundProject:国家自然科学基金（31270897，81271682，31770913，81771947）；江苏省高校优势学科（PAPD） |
| Author Name | Affiliation | E-mail | | Shang Jun | School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China | | | Xie Ting | School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China | | | Yin Narui | School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China | | | Chen Fei | School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China | | | Du Jie | School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China | | | Zhang Haowen | School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China | | | Yu Jiahua | School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China | | | Liu Fenju | School of Radiation Medicine and Protection, Medical College of Soochow University, State Key Laboratory of Radiation Medicine and Protection, Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China | fangsh@suda.edu.cn |
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| Abstract:: |
| Objective To investigate the effects of titanium dioxide (TiO2) nanoparticles coupled with nuclear localization sequence (NLS) on the radiosensitivity of U251 glioma cells. Methods Synthesis and characterization of the TiO2-NLS nanoparticles with nuclear targeting property. U251 cells were treated with nanoparticles and/or ionizing radiation. Flow cytometry analysis was performed to measure the ROS content and the cell apoptotic percentage. The DNA damage was detected by using γ-H2AX foci staining. Clonogenic survival assay was used to evaluate the radiosensitivity of U251 cells. Results NLS modification promoted nuclear translocation of TiO2nanoparticles. Compared with the control nanoparticles, TiO2-NLS treatment increased radiation-induced cell apoptosis (t=8.96, P<0.05). Clonogenic survival assay showed the radiosensitization ratios of TiO2 nanoparticle-treated group and TiO2-NLSnanoparticle-treated group were 1.18 and was 1.29, respectively. These two ratios had statistically difference (t=14.72, P<0.05). Conclusions Nuclear targeting TiO2 nanoparticles enhances the radiosensitivity of U251 glioma cells. |
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