| Kong Feifei,Li Yuanjie,Ma Lu,et al.Effect of Rev1 siRNA on the sensitivity of human colon cancer cells to X-ray radiation[J].Chinese Journal of Radiological Medicine and Protection,2018,38(10):721-727 |
| Effect of Rev1 siRNA on the sensitivity of human colon cancer cells to X-ray radiation |
| Received:June 04, 2018 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.10.001 |
| KeyWords:Colon cancer cells Rev1 X-rays Radiosensitivity |
| FundProject:国家自然科学基金（31360251）；宁夏回族自治区重点研发计划项目（宁科技字[2016]25号） |
| Author Name | Affiliation | E-mail | | Kong Feifei | Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan 750004, China | | | Li Yuanjie | Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan 750004, China | | | Ma Lu | Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan 750004, China | | | Sui Yu | Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan 750004, China | | | Zhe Hong | Department of Radiotherapy, Cancer Hospital, General Hospital of Ningxia Medical University, Yinchuan 750004, China | | | Xu Fang | Key Laboratory of Fertility Preservation and Maintenance of the Ministry of Education, Ningxia Medical University, Yinchuan 750004, China | xufang@nxmu.edu.cn |
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| Abstract:: |
| Objective To explore the effect of silencing Rev1 gene on the proliferation and apoptosis induction of human colon cancer cell line THC8307 after X-ray irradiation. Methods Rev1 siRNA was transferred into THC8307 cells and its transferring efficiency was tested by RT-PCR and Western blot assay. The cells were divided into blank control group, negative control group and radiation group. After 6 Gy irradiation, cell proliferation were detected by MTT assay, cell apoptosis were detected by flow cytometry (FCM), and the expressions of PCNA, γ-H2AX, P53, Bax, and Bcl-2 proteins in each group were detected by Western blot. Results Compared with the blank control group, after 6 Gy irradiation, the proliferation rate of the silenced Rev1 was reduced (t=7.53, P<0.05) and apoptosis rate was increased (t=6.23, P<0.05). The expression of PCNA and Bcl-2 decreased (t=4.39, 6.13 P<0.05), and the expressions of γ-H2AX, P53 and Bax increased (t=5.48, 5.09, 3.32, P<0.05). Conclusions Silencing Rev1 gene inhibits the proliferation of colon cancer cells, promotes apoptosis, and increases cell radiosensitivity to X-rays. |
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