| Fu Guoxiang,Li Yuanchuang,Pan Mengxue,Guo Qiang,Rao Xinxin,Xu Xiaoya,Zhou Yi,Shao Chunlin,Gao Jianjun,Hua Guoqiang.Establishment of an intestinal organoid-based method to investigate the radiation mitigation effect of Epiregulin[J].Chinese Journal of Radiological Medicine and Protection,2018,38(9):641-646 |
| Establishment of an intestinal organoid-based method to investigate the radiation mitigation effect of Epiregulin |
| Received:March 29, 2018 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.09.001 |
| KeyWords:Intestinal stem cell Intestinal Organoid Radiation Epiregulin Radiation-induced gastrointestinal syndrome |
| FundProject:国家自然科学基金（31470826，31670858） |
| Author Name | Affiliation | E-mail | | Fu Guoxiang | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Li Yuanchuang | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Pan Mengxue | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Guo Qiang | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Rao Xinxin | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Xu Xiaoya | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Zhou Yi | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Shao Chunlin | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Gao Jianjun | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | | | Hua Guoqiang | Institute of Radiation Medicine, Fudan University, Shanghai 200032, China | guoqianghua@fudan.edu.cn |
|
| Hits: 3583 |
| Download times: 1577 |
| Abstract:: |
| Objective To establish an intestinal organoid-based assay to investigate the radiation mitigation effect of epiregulin in vitro. Methods Intestinal crypts were released from tissue incubated with EDTA. Intestinal crypts seeded in 3D matrigel were irradiated at 24 h after plating. The radiation mitigation effect of epiregulin was evaluated by measuring the survival rate, size and budding numbers of the organoid after irradiation, and the basic FGF was used as a positive control of epiregulin. Results Radiation-induced lethality and dose-dependent survival curve of the intestinal organoid were consistent with in vivo data. Treatment with epiregulin (400 ng/ml) at 24 h post-radiation significantly increased survival rate of 8 Gy X-ray irradiated intestinal organoid in comparison with non-treated group[(12.56±1.02)% vs. (4.73±0.38)%, t=12.43,P<0.05]. Conclusions Epiregulin has radiation mitigation effect on intestinal organoid and could serve as a potential medical countermeasure to mitigate gastrointestinal toxicity. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
