| Fang Zheng,Yuan Huifang,Zhao Junfang,et al.Effects of EZH2 siRNA on apoptosis induction and radiosensitivity of tongue cancer Tca-8113 cells[J].Chinese Journal of Radiological Medicine and Protection,2018,38(8):561-566 |
| Effects of EZH2 siRNA on apoptosis induction and radiosensitivity of tongue cancer Tca-8113 cells |
| Received:January 27, 2018 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.08.001 |
| KeyWords:Tongue squamous cell carcinoma Radiotherapy Apoptosis EZH2 |
| FundProject:国家自然科学基金（8140100298） |
| Author Name | Affiliation | E-mail | | Fang Zheng | Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Yuan Huifang | Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Zhao Junfang | Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | junfangzhao@zzu.edu.cn | | Sun Minglei | Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Qiao Bin | Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Wu Dapeng | Department of Radiotherapy, Huaihe Hospital of Henan University, Kaifeng 475000, China | | | Li Xinming | Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | | Sun Qiang | Department of Oral and Maxillofacial Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | |
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| Abstract:: |
| Objective To investigate the effect of EZH2 on apoptosis and radiosensitivity of squamous cell carcinoma of tongue.Methods Tongue squamous carcinoma cells Tca-8113 were transfected with small interfering RNA of EZH2 (EZH2 siRNA1, EZH2 siRNA2) and its negative controls (siRNA-NC), the expression levels of EZH2 were detected by RT-PCR and Western blot and EZH2 siRNA2 was used for further studied since its better interference efficiency. The cells with siRNA transfection were irradiated with 8 Gy doses, and cell proliferation was detected by MTT, apoptosis was detected by flow cytometry, the expression of p-STAT3, STAT3 and Cleaved Caspase-3 was detected by Western blot. In addition, the cells were irradiated with 0, 2, 4, 6, and 8 Gy to detect radiosensitivity by cell colony formation assay.Results EZH2 siRNA1 and EZH2 siRNA2 decreased the expression of EZH2 in Tca-8113 cells and EZH2 siRNA2 had a better interference efficiency (tmRNA=8.660, PmRNA<0.01; tprotein=2.883, Pprotein<0.05). The apoptotic rate in the EZH2 siRNA group was (29.90±1.64)%, and was increased by 8 Gy irradiation to (38.17±1.59)% (t=4.742, P<0.05). At the same time, EZH2 siRNA reduced the level of p-STAT3, but promoted the expression of Cleaved Caspase-3 protein, and enhanced the sensitivity of Tca-8113 cells to 1.668-times of control.Conclusions Interfering EZH2 could promote apoptosis, inhibit proliferation and increase radiosensitivity of squamous cell carcinoma of tongue. |
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