| Ma Ji,Ye Caiyong,Ding Nan,et al.DNA-PKcs functions in cellular hyper-radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2018,38(7):489-493 |
| DNA-PKcs functions in cellular hyper-radiosensitivity |
| Received:March 13, 2018 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.07.002 |
| KeyWords:Tumor cells Low dose hyper-radiosensitivity Chk2 kinase DNA-PKcs |
| FundProject:国家自然科学基金（11405235） |
| Author Name | Affiliation | E-mail | | Ma Ji | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | | | Ye Caiyong | Department of Space Radiobiology, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | | Ding Nan | Department of Space Radiobiology, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | | Zhu Jiayun | Department of Space Radiobiology, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China | | | Hu Wentao | School of Radiation Medicine and Protection, Medical College of Soochow University, Suzhou 215123, China | wthu@suda.edu.cn |
|
| Hits: 6247 |
| Download times: 2545 |
| Abstract:: |
| Objective To explore the functions of DNA-PKcs in cellular low dose hyper-radiosensitivity. Methods Colony-formation assay was used to detect the survival fractions of M059K and M059J cell lines treated by X-ray irradiation. Micronucleus assay and γ-H2AX foci assay were used to measure the radiation-induced DNA damage. Western blot was used to detect the relative expression levels of phospho-Chk1, total Chk1, phospho-Chk2 and total Chk2 of M059K and M059J cells. Results The hyper-radiosensitivity was observed in M059K cells irradiated with X-ray of doses lower than 1 Gy. DNA damage levels did not show HRS/IRR in the cell lines we used. pChk1/Chk1 in M059K cells was significantly increased during 20 min to 60 min after 0.2 Gy X-ray irradiation (t=14.157, 13.661, 14.177, 11.317, 14.512, P<0.05); pChk2/Chk2 in M059K cells was markedly increased during 20 min to 50 min after 0.2 Gy X-ray irradiation (t=13.182, 13.868, 14.155, 14.477, P<0.05). Conclusions M059K cells show the phenomenon of low dose hyper-radiosensitivity, which may be related to activation of proteins in G2/M phase checkpoints regulated by DNA-PKcs. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
