| Nai Aitao,Wang Zhen,Cao Wenyu,Xu Yang,Liu Zhenghai,He Jie,Chen Xi,He Shuya,Ai Xiaohong,Wan Wei.The protective effect of environmental enrichment on radiation induced cognitive dysfunction and underlying mechanism[J].Chinese Journal of Radiological Medicine and Protection,2018,38(6):401-406 |
| The protective effect of environmental enrichment on radiation induced cognitive dysfunction and underlying mechanism |
| Received:January 12, 2018 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.06.001 |
| KeyWords:Ionizing radiation Cognitive dysfunction Environmental enrichment Microglial activation Synapse |
| FundProject: |
| Author Name | Affiliation | E-mail | | Nai Aitao | Department of Radiation Oncology, The First Affiliated Hospital of University of South China, Hengyang 421001, China | | | Wang Zhen | Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang 421001, China | | | Cao Wenyu | Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang 421001, China | | | Xu Yang | Department of Physiology, Medical College, University of South China, Hengyang 421001, China | | | Liu Zhenghai | Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang 421001, China | | | He Jie | Department of Pathology, Medical College, University of South China, Hengyang 421001, China | | | Chen Xi | Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang 421001, China | | | He Shuya | School of Public Health, University of South China, Hengyang 421001, China | | | Ai Xiaohong | Department of Radiation Oncology, The First Affiliated Hospital of University of South China, Hengyang 421001, China | | | Wan Wei | Clinical Anatomy & Reproductive Medicine Application Institute, Medical College, University of South China, Hengyang 421001, China | david-wan@163.com |
|
| Hits: 4081 |
| Download times: 1716 |
| Abstract:: |
| Objective To investigate the protective effect and mechanism of environmental enrichment (EE) on radiation induced cognitive dysfunction in mice. Methods A total of 45 female Kunming mice (2-month old) were randomly divided into control group, irradiation group and irradiation plus EE group with 15 in each group. Irradiation group and irradiation plus EE group were treated with a single dose of 4 Gy whole body irradiation, irradiation plus EE group were housed in EE condition for 35 d after irradiation. The object recognition task was used to evaluate the cognitive function of mice. The expression of microglial marker IBA-1 in hippocampus was determined by immunohistochemical staining. The expressions of CD68 and synaptophysin (SYP) proteins in hippocampus were assayed by Western blot. Results Compared with control group, the irradiation group had a low discrimination ratio in object recognition task and had a remarkable low level of SYP expression in hippocampus (t=3.66, 6.84, P<0.05). In addition, radiation activated microglia in hippocampus by increasing the number of IBA-1-positive cells and enhancing the expression of CD68 (t=6.83, 5.79, P<0.05). Compared with irradiation group, irradiation plus EE group increased the discrimination ratio and the expression of SYP in hippocampus (t=3.56, 4.06, P<0.05), while the number of IBA-1-positive cells and the expression of CD68 were significantly reduced (t=7.69, 4.59, P<0.05). Conclusions A single dose of 4 Gy whole body irradiation leads to cognitive dysfunction in mice, while EE could effectively improve the animals' cognitive behavior possibly by inhibiting microglial activation and preventing synapse loss in hippocampus. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
