| Wang Jinhan,Lu Qianying,Ji Kaihua,Du Liqing,Wang Yan,Xu Chang,Liu Qiang.The dynamic changes of gene expressions in radiation-injured intestine based on RNA-sequence analysis[J].Chinese Journal of Radiological Medicine and Protection,2018,38(2):81-86 |
| The dynamic changes of gene expressions in radiation-injured intestine based on RNA-sequence analysis |
| Received:September 25, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.02.001 |
| KeyWords:RNA-seq Radiation-induced intestinal injury Differential expression genes Signaling pathways Gene interaction network |
| FundProject:国家自然科学基金（31670859） |
| Author Name | Affiliation | E-mail | | Wang Jinhan | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Insititute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | Lu Qianying | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Insititute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | Ji Kaihua | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Insititute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | Du Liqing | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Insititute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | Wang Yan | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Insititute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | Xu Chang | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Insititute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | | | Liu Qiang | Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Insititute of Radiation Medicine, Chinese Academy of Medical Science, Peking Union Medical College, Tianjin 300192, China | liuqiang@irm-cams.ac.cn |
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| Abstract:: |
| Objective To investigate the dynamic changes of gene expressions in mouse jejunum after lethal dose abdomen irradiation(ABI). Methods RNA was extracted from mouse jejunum at 0 and 6 h, 3.5 and 5 d after 14 Gy 137Cs γ-ray ABI and then subjected to RNA-sequence analysis. Gene with expressions changed more than 2-fold of control were identified as differentially expressed ones. The selected genes were subsequently analyzed using IPA, Funrich, GO and KEGG software. Results Gene analysis of mouse jejunum samples showed that radiation activated p53 pathway at 6 h and 3.5 d after ABI. Interaction network analysis of genes suggested that Lck, Cdk1 and Fyn, genes could play an important role in jejunum damage at 3.5 d after ABI. The gene expression profiles demonstrated that ABI up-regulated DNA damage repair pathways and down-regulated cell adhesion molecules, focal adhesion and IgA production pathways. Conclusions The p53 signaling pathway and some key genes such as Lck, Cdk1, and Fyn may contribute to the radiation-induced intestinal injury. |
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