| Gao Yanxiao,Tian Mei,Gao Gang,Ruan Jianlei,Piao Chunnan,Wu Lina,Liu Jianxiang.Cell cycle and its regulatory proteins in the peripheral blood lymphocytes of the residents living in a radon hot spring area[J].Chinese Journal of Radiological Medicine and Protection,2018,38(1):12-16 |
| Cell cycle and its regulatory proteins in the peripheral blood lymphocytes of the residents living in a radon hot spring area |
| Received:August 14, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.01.003 |
| KeyWords:Radon Lymphocyte Cell cycle Regulatory proteins |
| FundProject: |
| Author Name | Affiliation | E-mail | | Gao Yanxiao | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Mei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Gao Gang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Ruan Jianlei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Piao Chunnan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Wu Lina | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Jianxiang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | liujianxiang@nirp.chinacdc.cn |
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| Abstract:: |
| Objective To observe potential effect of radon hot springs on the changes of cell cycle and its regulatory proteins of CDK1, CDK2, CDK4, CDK6,CyclinD1, CyclinE1, WEE1, CDC25A in the peripheral blood lymphocytes of residents. Methods A random sampling method was used to persons 46 persons from the residents around radon hot spring in Wentang town, and 39 persons were selected from the control area without radon exposure. Flow cytometry was used to detect the changes of cell cycle and the expressions of cell cycle-related regulatory proteins. Multiple linear regression method was used to analyze the relationship between the expressions of cell cycle regulatory proteins and radon exposure. Results The percentages of cells at G0/G1 phase and S phase in lymphocytes were different in the two groups (t=2.250, -2.382, P<0.05). The expression levels of CDK1, CDK6 and CyclinE1 in the peripheral blood lymphocytes of radon hot spring group were significantly decreased (t=4.770,11.419,5.238,P<0.05) and negatively correlated with radon exposure (t=-5.097, -11.128,-5.117, P<0.05).The expression levels of CDK2, CDK4, CyclinD1, WEE1 and CDC25A in the peripheral blood lymphocytes of radon hot spring group were increased but not significantly(P>0.05). Conclusions The incidences of a higher ratio of S-phase cells and lower expression levels of CDK1, CDK6 and CyclinE1 in the peripheral blood lymphocytes of residents in Wentang town may be related to long-term radon exposure. |
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