| Su Xiaoyu,Xu Huiqin,Wang Hui,et al.Radiosensitization of irisquinone on the Warburg effect of MDA-MB231 cells[J].Chinese Journal of Radiological Medicine and Protection,2018,38(1):1-5 |
| Radiosensitization of irisquinone on the Warburg effect of MDA-MB231 cells |
| Received:June 15, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2018.01.001 |
| KeyWords:Irisquinone Radiosensitization Warburg effect HKⅡ |
| FundProject:国家自然科学基金（81371587） |
| Author Name | Affiliation | E-mail | | Su Xiaoyu | Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China | | | Xu Huiqin | Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China | hfxuhuiqin@163.com | | Wang Hui | Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China | | | Zhang Dan | Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China | | | Qiao Feng | Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China | | | Yu Wenjing | Department of Nuclear Medicine, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, China | |
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| Abstract:: |
| Objective To study the radiosensitization of irisquinone on the Warburg effect of MDA-MB231 cells. Methods MDA-MB231 cells in the exponential growth phase were divided into 6 groups:control group, irisquinone group, radiation group, irisquinone plus radiation group(Irisquinone+RA), negative control group, and experimental group (siRNA). Colony formation assay was used to measure cell survival fraction of MDA-MB231 cells. Single-hit multi-target model was used to fit the survival curve and calculate the sensitive enhancement ratio (SER). Flow cytometry was used to measure cell apoptosis. The relative expression levels of HK Ⅱ mRNA and protein in each group were detected by qRT-PCR and Western blot respectively. Results The values of D0, Dq and SF2 in the irisquinone plus radiation group were obviously lower than those in the radiation group. The SER of irisquinone was 1.52. Compared with the other groups, the cell apoptosis rate was increased (t=13.29,12.09,5.90,3.83, P < 0.05),while the relative expression levels of HK Ⅱ mRNA (t=9.14, 10.48, 3.40,P < 0.05) and protein (t=13.39, 16.08, 5.81,P < 0.05) were decreased in the irisquinone plus radiation group significantly. Conclusions Radiosensitization function of irisquinone inhibits the Warburg effect of MDA-MB231 cells by down-regulating the expression of HKⅡ. |
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