| Yang Ying,Li Zhipeng,Jia Chunli,et al.Mechanism of Coix seed injection enhances the radiosensitivity in hepatocellular carcinoma cell Bel-7402[J].Chinese Journal of Radiological Medicine and Protection,2017,37(12):885-890 |
| Mechanism of Coix seed injection enhances the radiosensitivity in hepatocellular carcinoma cell Bel-7402 |
| Received:May 27, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.12.001 |
| KeyWords:Radiosensitivity Coix seed injection X-rays Hepatoma cell |
| FundProject:国家自然科学基金（81560388） |
| Author Name | Affiliation | E-mail | | Yang Ying | Department of Cancer Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | | | Li Zhipeng | Department of Cancer Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | | | Jia Chunli | Department of Cancer Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | | | Liu Qiang | Department of Urology, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | | | Yang Zhifang | Department of Cancer Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | | | Zhang Ruili | Department of Cancer Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | | | Mao Rui | Department of Cancer Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | | | Zhang Hua | Department of Cancer Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | | | Aisiker·Tulahong | Department of Cancer Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | | | Bao Yongxing | Department of Cancer Center, First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China | baoyx@vip.sina.com |
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| Abstract:: |
| Objective To observe the effects of Coix seed injection on the cell viability and radiosensitivity of human hepatoma cell line Bel-7402. Methods Bel-7402 cells were irradiated by X-rays, or treated with Coix seed injection, or treated with both of them. The cells proliferation and apoptosis were detected by MTT and by flow cytometry respectively. Cell cloning was used to observe the number of viable cells and to draw the cell survival curve. The mRNA and protein level of Bax, Bcl-2 were detected by RT-PCR and Western blot respectively. Results It was found that the Coix seed injection group(12 μmol/L) and X-ray group(8 Gy)had a significant inhibitory effect on the growth of hepatocellular carcinoma cells(t=17.03, 11.26, P<0.05).And compared with Coix group and irradiation group, the combined treatment group showed higher inhibition rate(t=24.80, 20.19, P<0.05). The mRNA and protein levels of Bax were gradually elevated(F=437.92, 67.91,P<0.05), while the expressions of Bcl-2 reflected a decreased trend (F=31.18, 48.50,P<0.05). The D0 values of pure irradiation group and combined treatment group were 4.27 and 3.34, respectively, and the sensitization enhancement ratio was 1.27. Conclusions The Coix seed injection inhibit cell growth and induce apoptosis, as well as increase radiative sensitization may via the apoptosis related factors Bax and Bcl-2 in hepatocellular carcinoma. |
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