| Ni Meng,Yin Tao,Li Ming,et al.Programmed cell death 4 enhances radiosensitivity of pancreatic cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2017,37(11):810-815 |
| Programmed cell death 4 enhances radiosensitivity of pancreatic cancer cells |
| Received:April 07, 2017 Revised:April 07, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.11.002 |
| KeyWords:Pancreatic cancer Radiosensitivity Programmed cell death 4 β-catenin c-myc |
| FundProject:国家自然科学基金（81372665） |
| Author Name | Affiliation | E-mail | | Ni Meng | Department of Gastroenterology, Nanyang Hospital Affiliated to Zhengzhou University, Nanyang 473000, China | | | Yin Tao | Department of Pancreatic Surgery, Union Hospital Affiliated to Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430022, China(Yin T | | | Li Ming | Department of Oncology, Nanyang Hospital Affiliated to Zhengzhou University, Nanyang 473000, China | | | Yang Zheng | Department of Radiotherapy, Nanyang Hospital Affiliated to Zhengzhou University, Nanyang 473000, China | | | Liu Chi | Department of Liver Surgery, Nanyang Hospital Affiliated to Zhengzhou University, Nanyang 473000, China | | | Fan Hongwei | Department of Gastroenterology, Nanyang Hospital Affiliated to Zhengzhou University, Nanyang 473000, China | xhnkfhw@sohu.com |
|
| Hits: 3497 |
| Download times: 1968 |
| Abstract:: |
| Objective To investigate the effect and mechanism of programmed cell death 4 (PDCD4) on radiosensitivity of pancreatic cancer cells. Methods Pancreatic cancer tissues and corresponding adjacent tissues were collected, the expression level of PDCD4 was detected by RT-PCR and Western blot. Human pancreatic cancer cells Sw1990 were transfected with PDCD4 overexpression vector (group pIRES2-PDCD4), empty vector (pIRES2 group), and treated with transfection reagent, respectively. The expression level of PDCD4 was detected by RT-PCR and Western blot. After radiation treatment, cell apoptosis was detected by flow cytometry, cell survival was detected by clone assay, and the expression levels of β-catenin, c-myc and Cleaved Caspase-3 were detected by Western blot. Results The expression of PDCD4 mRNA and protein in pancreatic cancer tissues was significantly lower than that in adjacent tissues (t=4.869, 9.208, P<0.05). The expression of PDCD4 mRNA and protein in pIRES2-PDCD4 group was significantly lower than that in the non-transfection group (t=9.074, 18.927, P<0.05). After radiation, the apoptosis rate and Cleaved Caspase-3 level in the pIRES2-PDCD4 group were significantly higher than those in the non-transfection group (t=3.670, 4.086, P<0.05), while the expression levels of β-catenin and c-myc in the cells were significantly lower than those in the non-transfection group (t=9.242, 17.644, P<0.05). The radiosensitivity of pIRES2-PDCD4 group was higher than that of non-transfection group, and the sensitization ratio was 1.843. Conclusions PDCD4 can increase radiosensitivity and promote apoptosis of pancreatic cancer cells, to which the Wnt signaling pathway may be related. |
| HTML View Full Text View/Add Comment Download reader |
| Close |
|
|
|
