| Sun Fei,Sun Zhiqiang,Wang Jianlin,Wang Jian,Hu Lijun,Zhou Mengyun,Feng Yue,Kong Ze,Hua Qiu,Yu Jingping.Radiosensitizing effect of apatinib on esophageal cancer cell line Kyse-150[J].Chinese Journal of Radiological Medicine and Protection,2017,37(11):805-809,831 |
| Radiosensitizing effect of apatinib on esophageal cancer cell line Kyse-150 |
| Received:June 25, 2017 Revised:June 25, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.11.001 |
| KeyWords:Esophageal cancer Radiosensitivity Apatinib Apoptosis Cell cycle |
| FundProject:国家自然科学基金（11705095） |
| Author Name | Affiliation | E-mail | | Sun Fei | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | | | Sun Zhiqiang | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | | | Wang Jianlin | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | | | Wang Jian | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | | | Hu Lijun | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | | | Zhou Mengyun | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | | | Feng Yue | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | | | Kong Ze | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | | | Hua Qiu | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | | | Yu Jingping | Department of Radiotherapy, Changzhou No.2 People's Hospital, Affiliated Hospital of Nanjing Medical University, Changzhou 213003, China | yujingping700420@sina.com |
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| Abstract:: |
| Objective To evaluate the radiosensitization effect of apatinib on esophageal cancer cell line Kyse-150, and to investigate the underlying mechanism. Methods Cells were divided into four groups:control group, apatinib treatment group, X-ray radiation group, and the combination group treated with X-rays plus apatinib. The effect of apatinib with different concentrations on the cell proliferative and radiosensitivity were evaluated by CCK-8 kit and colony formation assay. Flow cytometry method was adopted to detect the effect of apatinib on cell cycle progress and apoptosis induction. Results Apatinib inhibited the proliferation of Kyse-150 cells in time-and dose-dependent manners (r=0.89-0.96, P<0.05). With the increase of apatinib concentration, D0, Dq and SF2 value of Kyse-150 cells decreased and SERD0 value increased. Compared with control group, apatinib alone group, and radiation alone group, the cell apoptosis rate significantly increased in the combination group (t=12.36, 5.99, 15.47,P<0.05). Compared with control group, the percentages of cells in G2/M phase were all significantly increased in apatinib group, radiation group and combination group (t=8.81, 39.69, 20.61,P<0.05). Compared with radiation alone group and control group, the percentage of cells in S phase significantly increased in apatinib alone group and combination group(t=6.06, 3.82,8.81,6.24,P<0.05). ConclusionsApatinib can increase radiosensitivity of esophageal cancer cell line Kyse-150 possibly by inhibiting cell proliferation, inducing cell apoptosis and causing redistribution of cell cycle. |
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