| Wang Ye,Xiong Yongchao,Xue Shaobo,Wu Zhihua,Peng Zhenjun.Attenuation of metformin on radiation-induced lung fibrosis in rats[J].Chinese Journal of Radiological Medicine and Protection,2017,37(10):736-741 |
| Attenuation of metformin on radiation-induced lung fibrosis in rats |
| Received:March 14, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.10.003 |
| KeyWords:Metformin Radiation-induced pulmonary injury Transforming growth factor-β Fibrosis |
| FundProject:湖北省自然科学基金（2017CFB770） |
| Author Name | Affiliation | E-mail | | Wang Ye | School of Pharmacy, Wuhan University, Wuhan 430071, China | | | Xiong Yongchao | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | Xue Shaobo | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | Wu Zhihua | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | | | Peng Zhenjun | Cancer Center, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China | 694188570@qq.com |
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| Abstract:: |
| Objective To evaluate the radioprotective effects of metformin on radiation-induced pulmonary injury in rats.Method A total of 30 Sprague-Dawley (SD) rats were randomly divided into three groups:control, radiation (20 Gy) and radiation (20 Gy) with metformin, with 10 rats in each group. The right lungs of rats were irradiated to 20 Gy with 6 MV X-rays. Computed tomography (CT) was performed and Hounsfield Units (HU) were determined during the observation period. The tissue samples of lung were extracted to perform the histological analysis, measurement of hydroxyproline content, fibrosis score and evaluation of fibrosis/inflammatory markers by Western blot at 12 weeks post-irradiation. CCK-8 method was used to explore the effects of metformin on non-small cell lung cancer(NSCLC) cells A549 and H460.Results Metformin reduced radiologic and histologic signs of fibrosis, lung density(6.52±0.43 vs. 3.31±0.57,t=6.37, P<0.01)and hydroxyproline content(32.58±1.59 vs. 23.47±2.46,t=12.72, P<0.01)which had been increased due to irradiation. Meanwhile, it significantly decreased the expressions of Col1, p-AMPKα and TGF-β, while inhibited the expressions of p-Smad2 and p-Smad3 compared to the irradiation alone group. Moreover, metformin reduced A549 and H460 cells growth.Conclusions Metformin exerted a protective effect on normal tissues in radiation-induced pulmonary fibrosis. Thus, it might act as a promising radioprotective agent in the treatment of lung cancer. |
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