| Guo Ya,Ma Hongbing,Wang Baofeng,Ren Hongtao.Bioinformatics analysis of radioresistance key gene and pathway in esophageal carcinoma[J].Chinese Journal of Radiological Medicine and Protection,2017,37(10):730-735 |
| Bioinformatics analysis of radioresistance key gene and pathway in esophageal carcinoma |
| Received:February 19, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.10.002 |
| KeyWords:Esophageal carcinoma Radioresistance Gene expression omnibus Bioinformatics |
| FundProject: |
| Author Name | Affiliation | E-mail | | Guo Ya | Department of Oncology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China | | | Ma Hongbing | Department of Oncology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China | mhbxian@126.com | | Wang Baofeng | Department of Oncology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China | | | Ren Hongtao | Department of Oncology, Second Affiliated Hospital of Medical College, Xi'an Jiaotong University, Xi'an 710004, China | |
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| Abstract:: |
| Objective To explore key gene and pathway of radioresistance in esophageal carcinoma and reveal its molecular mechanism of radioresistance.Method The gene expression profiles of GSE61772 and GSE61620 were downloaded from the Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) were screened by GEO2R. Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (Kegg) enrichment and protein-protein interaction (PPI) network construction were performed by DAVID and String softwares. RT-PCR was used to detect the differences in the expression of different genes in different radiosensitivity cells.Results A total of 49 differentially expression genes were screened. These genes were mainly involved in the regulation of multicellular biosynthesis, ion transport, DNA synthesis, metabolism, cell proliferation and so on. The major biological pathways included a Wnt signal pathway. 12 DEGs interacted with each other, and CHN2 may be a key node. The expression of CHN2 gene had no obvious difference between TE13R and TE13.Conclusions 49 differentially expressed genes, including CHN2, may be involved in radioresistance of esophageal carcinoma, and the Wnt signaling pathway may be an important pathway in this radioresistance. |
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