Luo Ming,Cai Wangqing,Li Kaishu,Liu Jiahao,Xue Weiping,Liu Yimin.Effect of PDLIM4 gene on the prognosis of glioma and its cell radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2017,37(10):725-729,762
Effect of PDLIM4 gene on the prognosis of glioma and its cell radiosensitivity
Received:March 05, 2017  
DOI:10.3760/cma.j.issn.0254-5098.2017.10.001
KeyWords:PDLIM4  Glioma  Malignant degree  Prognosis  Radiation resistance
FundProject:广东省科技计划项目(2014A020212091)
Author NameAffiliation
Luo Ming Department of Radiotherapy, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 
Cai Wangqing Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 
Li Kaishu Department of Neurosurgery, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan 511500, China 
Liu Jiahao Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 
Xue Weiping Department of Radiotherapy, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 
Liu Yimin Department of Radiotherapy, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China 
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Abstract::
      Objective To investigate the effect of PDLIM4 (PDZ and LIM domain 4) gene on the prognosis and radiosensitivity of glioma.Methods The differentially expressed genes were analyzed by bioinformatics technique using GSE53733 gene chip. The expression of PDLIM4 protein was detected by Western blot. The effects of PDLIM4 gene on glioma prognosis and glioma cell radiosensitivity were studied by using real-time fluorescence quantitative PCR, siRNA, MTT and cell flow cytometry assays.Results PDLIM4 gene had the most significant differential expression in the chip (logFC=1.055 897, P<0.05). The PCR assay of 40 glioma cases in our hospital confirmed that expression of PDLIM4 showed obvious difference between high- and low-grade gliomas (t=4.44, P<0.05), which was correlated with the survival of patients (χ2=5.52, P<0.05). Moreover, PDLIM4 gene was involved in radioresistance of glioma cells (t=35.99, P<0.05).Conclusions PDLIM4 gene expression level correlates with malignant degree and prognosis of glioma and also contributes to cell radioresistance.
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