| Luo Ming,Cai Wangqing,Li Kaishu,et al.Effect of PDLIM4 gene on the prognosis of glioma and its cell radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2017,37(10):725-729,762 |
| Effect of PDLIM4 gene on the prognosis of glioma and its cell radiosensitivity |
| Received:March 05, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.10.001 |
| KeyWords:PDLIM4 Glioma Malignant degree Prognosis Radiation resistance |
| FundProject:广东省科技计划项目（2014A020212091） |
| Author Name | Affiliation | | Luo Ming | Department of Radiotherapy, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China | | Cai Wangqing | Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China | | Li Kaishu | Department of Neurosurgery, The Sixth Affiliated Hospital of Guangzhou Medical University, Qingyuan 511500, China | | Liu Jiahao | Department of Neurosurgery, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China | | Xue Weiping | Department of Radiotherapy, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China | | Liu Yimin | Department of Radiotherapy, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou 510120, China |
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| Abstract:: |
| Objective To investigate the effect of PDLIM4 (PDZ and LIM domain 4) gene on the prognosis and radiosensitivity of glioma.Methods The differentially expressed genes were analyzed by bioinformatics technique using GSE53733 gene chip. The expression of PDLIM4 protein was detected by Western blot. The effects of PDLIM4 gene on glioma prognosis and glioma cell radiosensitivity were studied by using real-time fluorescence quantitative PCR, siRNA, MTT and cell flow cytometry assays.Results PDLIM4 gene had the most significant differential expression in the chip (logFC=1.055 897, P<0.05). The PCR assay of 40 glioma cases in our hospital confirmed that expression of PDLIM4 showed obvious difference between high- and low-grade gliomas (t=4.44, P<0.05), which was correlated with the survival of patients (χ2=5.52, P<0.05). Moreover, PDLIM4 gene was involved in radioresistance of glioma cells (t=35.99, P<0.05).Conclusions PDLIM4 gene expression level correlates with malignant degree and prognosis of glioma and also contributes to cell radioresistance. |
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