| Liu Mingzhu,Fan Ruitai,Gu Hao,et al.Effect of berberine on radiosensitivity of cervical cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2017,37(8):581-586 |
| Effect of berberine on radiosensitivity of cervical cancer cells |
| Received:January 20, 2017 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.08.004 |
| KeyWords:Cervical cancer Berberine Radiosensitivity Signaling pathway Cell apoptosis |
| FundProject:福建省中青年骨干项目（2013-ZQN-ZD-8）；福建省自然科学基金（2016J01437） |
| Author Name | Affiliation | | Liu Mingzhu | Department of Traditional Chinese Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Fan Ruitai | Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Gu Hao | Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Wang Xinjie | Department of Traditional Chinese Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Liu Yanjie | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China |
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| Abstract:: |
| Objective To investigate the effect of berberine on the radiosensitivity of cervical cancer cells. Methods 5, 10, 15, 20 μmol/L of berberine were used to treat cervical cancer cell lines of Siha, HeLa, Caski. DMSO was applied as control of drug treatment. Cell proliferation was detected by the CCK-8 method, and then the half inhibitory concentration of berberine was calculated. Cell apoptosis and cell cycle distribution were detected by flow cytometry. Protein expressions of Cleaved Caspase-3, Cyclin B1, CDK1, STAT3 and p-STAT3 were detected by Western blot. Cervical cancer cells of Siha were treated by berberine with a half inhibitory concentration for 24 h and then irradiated with 0, 2, 4, 6, 8 Gy of X-rays. Cell clone assay was used to detect cell survival. Results Berberine could inhibit the growth of cervical cancer cells with a half inhibition concentration of(16.84±3.52),(23.54±8.67),(21.86±6.35)μmol/L for Siha, Caski, and HeLa cells, respectively. The berberine at 17 μmol/L could induce apoptosis(t=56.847,P<0.01)and G2/M phase arrest(t=47.251,P<0.01)in Siha cells, which also inhibited the expressions of Cyclin B1, CDK1 and p-STAT3 and promoted the expression of cleaved Caspase-3, but did not influence the expression of STAT3 in cervical cancer cells. Treatment of cells with 17 μmol/L berberine increased the radiosensitivity of cervical cancer cells with a sensitivity enhancement ratio of 1.55. Conclusions Berberine can inhibit cell proliferation, promote apoptosis, block cell cycle, and increase radiosensitivity of cervical cancer cells. |
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