Guo Ping,Wen Ling,Li Linlin,et al.Evaluation of the radiotherapy effect on transplanted breast cancer in mice by in vivo fluorescence imaging technique[J].Chinese Journal of Radiological Medicine and Protection,2017,37(6):408-414
Evaluation of the radiotherapy effect on transplanted breast cancer in mice by in vivo fluorescence imaging technique
Received:February 14, 2017  
DOI:10.3760/cma.j.issn.0254-5098.2017.06.002
KeyWords:Luciferase  Breast cancer  Lymph node metastasis  In vivo fluorescence imaging  X-ray
FundProject:
Author NameAffiliationE-mail
Guo Ping School of Radiation Medicine and Protection, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China  
Wen Ling School of Radiation Medicine and Protection, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China  
Li Linlin School of Radiation Medicine and Protection, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China  
Qiu Shanshan School of Radiation Medicine and Protection, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China  
Zhu Ran School of Radiation Medicine and Protection, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China  
Zeng Jianfeng School of Radiation Medicine and Protection, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China  
Zhang Youjiu School of Radiation Medicine and Protection, Medical College of Soochow University, Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection, Suzhou 215123, China yjzhang@suda.edu.cn 
Hits: 2584
Download times: 2185
Abstract::
      Objective To establish a mouse model of lymph node metastasis of breast cancer cells by luciferase imaging assay,to monitor early process of lymph node metastasis,and to evaluate the effect of X-ray radiation therapy on tumor. Methods The mouse mammary cancer cell line 4T1-Luc expressing luciferase was inoculated subcutaneously into the paw pad of nude mice to establish a model of subcutaneous lymph node metastasis.The lymph node metastasis in nude mice was continuously observed by in vivo fluorescence imaging system,and the nude mice with early lymph node metastasis of breast cancer cells were divided into control group and treatment group randomly.The radiotherapy effect was observed by in vivo fluorescence imaging system and evaluated by the pathological changes of HE staining of tumor tissue. Results A mouse lymph node metastasis model of breast cancer cells was successfully established,and the volume of primary tumor in paw correlated with the fluorescence photon number positively (r=0.958,P<0.001).On the twenty-fourth day after inoculation,the fluorescence photon number in pad tumor and popliteal fossa tumor of treatment group were significantly decreased in comparison with the control group (t=32.58,P<0.05),and the inhibition ratio of radiotherapy on tumor growth approached to 85%.HE staining showed that the apoptosis and necrosis in irradiated tumor was obviously higher than that in control group. Conclusions Bioluminescence imaging technique can be used to evaluate the effect of X-ray on breast cancer suppression and lymph node metastasis in mice.
HTML  View Full Text  View/Add Comment  Download reader
Close

Copyright©    Editorial Office of Chinese Journal of Radiological Medicine and Protection    

Beijing ICP No. 05020547 -2

Address: 2 Xinkang Street, Dewai, Beijing 100088, China

Telephone:010-62389620; Email:cjrmp@cjrmp.sina.net

Technical Support:Beijing E-tiller CO.,LTD.

Visitors:10225418  On-line:0

v
Scan QR Code
&et=D700F206953109B3D6C39329A22A8031D9343BB46AA6F1F10968521EE142DBC1943AF509CEA799267BD6ED130E6811DCDB548EFB8A93C91072AC09FB4AB3B9D05689A8455B5775587F97A9DAC3A104B558B917272ABC9044B53349B8A107CBAB02C85D4CD04DF05A074D29C97C656AA00BEFE307F6D2729526765E8390120EFD&pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=D4D466D60FDC1A5A&jid=5E4353813E091AB841B02B880782B82C&yid=FA004A8A4ED1540B&aid=6DF7CB66686A5806D8544C416F6AB494&vid=&iid=B31275AF3241DB2D&sid=A53D7AA35F9929AF&eid=3081401A9FAB9CE2&fileno=20170602&flag=1&is_more=0"> var my_pcid="A9DB1C13C87CE289EA38239A9433C9DC"; var my_cid="D4D466D60FDC1A5A"; var my_jid="5E4353813E091AB841B02B880782B82C"; var my_yid="FA004A8A4ED1540B"; var my_aid="6DF7CB66686A5806D8544C416F6AB494";