| Si Shaoyan,Wang Zongye,Lyu Guangming,Song Shujun,Liu Yanjun,Qin Yaya,Wu Yingying,Yan Chunhua.Effects of cerium oxide nanoparticles on the distribution of immune cells in peripheral blood and the proliferation of spleen T lymphocyte in X-ray irradiated mice[J].Chinese Journal of Radiological Medicine and Protection,2017,37(5):339-344 |
| Effects of cerium oxide nanoparticles on the distribution of immune cells in peripheral blood and the proliferation of spleen T lymphocyte in X-ray irradiated mice |
| Received:November 27, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.05.004 |
| KeyWords:Cerium oxide nanoparticles Irradiation injury Immune cells T lymphocytes Cell proliferation |
| FundProject:国家自然科学基金（11505012，81472814）；北京市自然科学基金（7172048，1174016）；北京市医院管理局“青苗”计划专项经费（QML20151004）；质检公益性行业科研专项（201510001-02） |
| Author Name | Affiliation | E-mail | | Si Shaoyan | Center for Special Medicine and Experimental Research, Department of Radiation Oncology, Diabetes Center, the 306th Hospital of PLA, Beijing 100101, China | | | Wang Zongye | Department of Radiation Oncology, the 306th Hospital of PLA, Beijing 100101, China | | | Lyu Guangming | Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China | | | Song Shujun | Center for Special Medicine and Experimental Research, Department of Radiation Oncology, Diabetes Center, the 306th Hospital of PLA, Beijing 100101, China | | | Liu Yanjun | Diabetes Center, the 306th Hospital of PLA, Beijing 100101, China | | | Qin Yaya | Center for Special Medicine and Experimental Research, Department of Radiation Oncology, Diabetes Center, the 306th Hospital of PLA, Beijing 100101, China | | | Wu Yingying | Center for Special Medicine and Experimental Research, Department of Radiation Oncology, Diabetes Center, the 306th Hospital of PLA, Beijing 100101, China | | | Yan Chunhua | Beijing National Laboratory for Molecular Sciences, State Key Laboratory of Rare Earth Materials Chemistry and Applications, PKU-HKU Joint Laboratory in Rare Earth Materials and Bioinorganic Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China | yan@pku.edu.cn |
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| Abstract:: |
| Objective To investigate protective effect of cerium oxide nanoparticles on immune system of X-ray irradiated mice.Methods Mice were randomly divided into 7 groups according to body weight layer: control group, irradiation group, and 5 different doses of cerium oxide nanoparticles administration groups. The mice of irradiation group and cerium oxide nanoparticles administration groups were irradiated once with 4 Gy X-rays. The mice of cerium oxide nanoparticles administration groups began to be intraperitoneally administrated with 10 ng, 100 ng, 1 μg, 10 μg and 100 μg cerium oxide nanoparticles per kilogram body weight twice a week on the fourth day before irradiation and these mice were killed on the tenth day after irradiation. White cells count and classification, percentages of T, B, NK, CD4+ and CD8+ T cells, and T lymphocyte proliferation of spleen were respectively analyzed by hymocytometer, flow cytometry and MTT assay. The ratios of CD4+ to CD8+ cells were calculated. Results Compared with control group, the numbers of leukocytes and NK cells, numbers and percentages of neutrophil granulocytes, monocytes, total lymphocytes, T lymphocytes, CD4+ and CD8+ T lymphocytes,spleen T lymphocytes proliferation were significantly decreased(t=2.26-3.18, P<0.05), and the percentage of B lymphocytes and ratio of CD4 to CD8 were significantly increased in irradiated mice(t=2.45, 3.18, P<0.05). Above immunology parameters were improved in irradiated mice after intraperitoneally administration with different dose of cerium oxide nanoparticles, especially 10 and 100 μg/kg. The radiation protective effect of 10 μg/kg cerium oxide nanoparticles was the best. Conclusions Cerium oxide nanoparticles can improve the immune function in irradiated mice. |
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