| Sun Chaonan,Qiao Qiao,Li Guang,Han Chuyang,Han Ning,Zhang Miao.Endoplasmic reticulum stress inhibitor salubrinal enhanced radiosensitivity of head and neck carcinoma cells[J].Chinese Journal of Radiological Medicine and Protection,2017,37(3):177-181 |
| Endoplasmic reticulum stress inhibitor salubrinal enhanced radiosensitivity of head and neck carcinoma cells |
| Received:October 17, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.03.003 |
| KeyWords:Head and neck squamous carcinoma Radiosensitivity Endoplasmic reticulum stress pathway inhibitor Glucose regulated proteins 78 |
| FundProject:国家自然科学基金（81402521） |
| Author Name | Affiliation | E-mail | | Sun Chaonan | Department of Radiotherapy, the First Hospital of China Medical University, Shenyang 110001, China | | | Qiao Qiao | Department of Radiotherapy, the First Hospital of China Medical University, Shenyang 110001, China | | | Li Guang | Department of Radiotherapy, the First Hospital of China Medical University, Shenyang 110001, China | 13804058616@163.com | | Han Chuyang | Department of Radiotherapy, the First Hospital of China Medical University, Shenyang 110001, China | | | Han Ning | Department of Radiotherapy, the First Hospital of China Medical University, Shenyang 110001, China | | | Zhang Miao | Department of Radiotherapy, the First Hospital of China Medical University, Shenyang 110001, China | |
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| Abstract:: |
| Objective To explore the effect of salubrinal (sal, an endoplasmic reticulum stress inhibitor) on radiosensitivity of human head and neck squamous carcinoma cells (HNSCC). Methods Cells were divided into two groups of sal treatment and its control. For drug treatment group, cells were treated with 10 mmol/L sal for different time (12, 24, 36 h) and then irradiated. The levels of a core protein GRP78 of endoplasmic reticulum stress (ERS) in HNSCC(KB, Fadu, and Detroit 562 cells)were analyzed by Western blot assay at different time (0, 20 min,1 h, 3 h, 6 h, 12 h, 24 h and 48 h) after irradiation. Cell survival was measured with colony formation assay. Results Western blot assay revealed that the protein levels of GRP78 in three kinds of HNSCC significantly increased from 20 min to 1 h and peaked at 3 h after radiation (t=12.72, 13.37, 18.31, P<0.05). Compared with the control group, treatment of cells with sal decreased GRP78 protein levels (t=14.25, 5.34, 3.12, P<0.05) in three cell lines and also significantly enhanced radiation damage and reduced cell viability. The sensitization enhancement ratios (SER) of sal in three cell lines were 1.16, 1.05 and 1.06, respectively. Conclusions Rradiosensitivity of HNSCC could be effectively enhanced by sal treatment. |
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