| Liu Yanjie,Gu Hao,Qiao Yuhuan,et al.Effect of silencing Chk1 expression on radiosensitivity of human cervical carcinoma HeLa cells[J].Chinese Journal of Radiological Medicine and Protection,2017,37(2):102-106 |
| Effect of silencing Chk1 expression on radiosensitivity of human cervical carcinoma HeLa cells |
| Received:July 03, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.02.004 |
| KeyWords:RNA interference Checkpoint kinase 1 Cervical cancer Radiosensitivity |
| FundProject: |
| Author Name | Affiliation | | Liu Yanjie | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Gu Hao | Department of Radiotherapy, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Qiao Yuhuan | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Shi Huirong | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Zhang Mengzhen | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Bian Aiping | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Ji Mei | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Guo Hongjun | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Chang Qing | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China | | Guo Ruixia | Department of Gynaecology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China |
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| Abstract:: |
| Objective To study the effect of checkpoint kinase 1(Chk1) knock-down on radiosensitivity of human cervical carcinoma HeLa cells.Methods Chk1 was knock-down by using a specific siRNA of Chk1 gene. After transfection with si-CHK1 or its negative control si-NC, HeLa cells were irradiated with 0, 2, 4, 6, 8 and 10 Gy of X-rays. The expressions of Chk1 mRNA and protein in irradiated HeLa cells were determined by RT-qPCR and Western blot, respectively. Cell proliferation was measured by MTT assay, apoptosis was measured by flow cytometry assay, radiosensitivity was determined by colony formation, and the protein expressions of P21 and Bcl-2 were determined by Western blot. Results After transfection of si-Chk1, both mRNA and protein levels of Chk1 in HeLa cells were decreased(t=2.12-5.86, P<0.05). Chk1 knock-down decreased cell proliferation(t=3.15-6.57, P<0.05), reduced colony formation ability(t=1.58-6.36, P<0.05), increased the protein expression level of P21(t=4.35, P<0.05)and decreased the protein level of Bcl-2(t=2.37, P<0.05)in the irradiated HeLa cells in comparison with nonirradiated control.Conclusions siRNA-mediated knock-down of Checkpoint kinase 1 enhances the radiosensitivity of HeLa cells. |
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