| Sun Ge,Qin Xiujun,Yin Jingjing,Zhang Wei,Huang Liqun,An Quan,Li Jianguo.Alteration of gene expression profile in Beagle dogs' peripheral blood lymphocytes after acute γ-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2017,37(2):88-95 |
| Alteration of gene expression profile in Beagle dogs' peripheral blood lymphocytes after acute γ-ray irradiation |
| Received:August 29, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.02.002 |
| KeyWords:γ-ray Beagle dog Lymphocytes Gene expression Pathway |
| FundProject:山西省实验动物专项资金（2014k09，2013k08） |
| Author Name | Affiliation | E-mail | | Sun Ge | Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Division of Radiology and Environmental Medicine of China Institute for Radiation Protection, Taiyuan 030006, China | | | Qin Xiujun | Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Division of Radiology and Environmental Medicine of China Institute for Radiation Protection, Taiyuan 030006, China | | | Yin Jingjing | Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Division of Radiology and Environmental Medicine of China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Wei | Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Division of Radiology and Environmental Medicine of China Institute for Radiation Protection, Taiyuan 030006, China | | | Huang Liqun | Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Division of Radiology and Environmental Medicine of China Institute for Radiation Protection, Taiyuan 030006, China | | | An Quan | Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Division of Radiology and Environmental Medicine of China Institute for Radiation Protection, Taiyuan 030006, China | | | Li Jianguo | Shanxi Key Laboratory of Drug Toxicology and Drug for Radiation Injury, Division of Radiology and Environmental Medicine of China Institute for Radiation Protection, Taiyuan 030006, China | ljg2547@163.com |
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| Abstract:: |
| Objective To investigate the changes of gene expression profile in Beagle dogs' peripheral blood lymphocytes after acute irradiation.Methods Totally 20 male adult Beagle dogs were randomly divided into four groups including non-treatment blank control group and three radiation groups exposed to 0.5, 2.0, and 5.0 Gy of γ-rays, respectively. Six hours after radiation, the peripheral blood were collected for lymphocytes isolation. Total RNA was extracted from the lymphocytes and analyzed by microarray hybridization. The differential gene profiles of radiation groups were analyzed by gene ontology (GO) analysis and kyoto encyclopedia of genes and genomes (KEGG) pathways analysis, and the alerted genes were further confirmed by the real time quantitative PCR (qRT-PCR) assay. Results Compared to the blank control group, the expressions of 308 genes in the radiation groups were perturbed over 2-fold of control, including 61 genes up-regulated and 247 genes down-regulated, which were mainly associated with immune response and cancer occurrence. The GO analysis indicated that the differential expressed genes were associated with cell connection, signal transduction, oxidation-reduction reaction and metabolism. The KEGG pathway analysis showed that some physiological and biochemical processes were involved, such as phagocytosis, tumor pathway, p53 signaling pathway, JAK-STAT signal pathway, cell differentiation, and proliferation, oxidative phosphorylation, glycogen dysplasia and other pathways. The microarray data of the alterations of apoptosis enhancing nuclease(AEN) and mitogenactivated protein kinase 13(MAP3K13) gene expressions were further confirmed by qRT-PCR. Conclusions Exposure to different doses of acute γ-ray irradiation had significant impact on the gene expressions in Beagle dogs' peripheral blood lymphocytes and those differential expressed genes were related to a series of biological processes and pathways including immune response, metabolism and carcinogenesis. |
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