| Wu Lina,Piao Chunnan,Tian Mei,et al.Effect of interference of SNCG gene on radiosensitivity of breast cancer T47D cells[J].Chinese Journal of Radiological Medicine and Protection,2017,37(1):19-23 |
| Effect of interference of SNCG gene on radiosensitivity of breast cancer T47D cells |
| Received:August 24, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2017.01.004 |
| KeyWords:Breast cancer γ-synuclein(SNCG) Radiosensitivity Gene therapy |
| FundProject: |
| Author Name | Affiliation | E-mail | | Wu Lina | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Piao Chunnan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Mei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Ruan Jianlei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Liu Jianxiang | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | jxliu@163.com |
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| Abstract:: |
| Objective To explore the role of γ-synuclein(SNCG) siRNA in the radiosensitivity of breast cancer T47D cells.Methods SNCG siRNA was synthesized according to the coding sequence of SNCG mRNA and then transiently transferred into T47D cell with lipofectamine. The expression of SNCG gene and protein was detected by RT-PCR and Western-blot, respectively. Cells were divided into three groups, SNCG siRNA interference group, negative control group and blank control group, which were irradiated with different doses of 60Co γ-rays. Cell radiosensitivity was evaluated by colony formation assay, cell proliferation was assayed by CCK-8 kit, and the protein expressions of phosphorylated-AKT and mTOR were detected by Western blot assay. Results Compared with blank control cells, the expressions of SNCG gene and protein in the SNCG siRNA transferred T47D cells were efficiently diminished. Cell colony formation results showed that, under 4, 6, 8 Gy irradiation, the cell survival of siRNA transfection group was lower than that of control group (t=5.449, 8.882, 21.503, P<0.05). CCK-8 experiments showed that the cell proliferation abilities of siRNA group at 24, 48, 72 h after 6 Gy irradiation were lower than those of control group (t=5.603, 4.839, 6.115, P<0.05). In addition, after 6 Gy irraddaition, the AKT and mTOR phosphorylation levels in the siRNA group were more obviously reduced compared with blank groups, but the total AKT and mTOR had no changes.Conclusions Transfection of SNCG siRNA can enhance the radiosensitivity of breast cancer cells probably by inhibiting p-AKT signal pathway. |
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