| Zhang Bin,Liu Dongmei,Ma Yanhui,et al.Over-expression of miR-141 increases the radiosensitivity of esophagus cancer cells[J].Chinese Journal of Radiological Medicine and Protection,2016,36(12):893-897 |
| Over-expression of miR-141 increases the radiosensitivity of esophagus cancer cells |
| Received:July 07, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.12.003 |
| KeyWords:miR-141 Esophagus cancer KYSE-150 cells Radiosensitivity |
| FundProject: |
| Author Name | Affiliation | | Zhang Bin | Department of Pharmacy, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450003, China | | Liu Dongmei | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450003, China | | Ma Yanhui | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450003, China | | Gao Na | Department of Pharmacy, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450003, China | | He Chunyu | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450003, China | | Zhao Xiuli | Department of Pharmacy, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450003, China | | Zhang Jingwei | Department of Radiation Oncology, Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou 450003, China |
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| Abstract:: |
| Objective To investigate the role and potential molecular mechanism of the radiosensitivity of esophagus cancer cells. Methods miR-141 mimics or its negative control was transfected into esophagus cancercells KYSE-150, respectively. Radiosensitivity of esophagus cancer cells was determined by CCK-8, flow cytometry and colony formation assay. The expression level of miR-141 was determined by qRT-PCR. Western blot was used to detect the expressions of proliferation-related protein Ki67 and apoptosis-related proteins Bax and Bcl-2. Results After radiation, the expression of miR-141 was decreased in KYSE-150 cells in a dose-dependent manner (t=2.57-8.96, P<0.05). Up-regulation of miR-141 significantly suppressed cell proliferation and colony formation and promoted apoptosis, indicating that overexpression of miR-141 enhanced the radiosensitivity of KYSE-150 cells(t=3.24, P<0.05). In addition, the miR-141 mimic significantly reduced the expressions of Ki67 and Bcl-2 protein (t=6.56, 8.24, P<0.01) and inhibited the expression of Bax protein compared with miR-control group (t=3.24, P<0.01). Conclusions Over-expression of miR-141 enhances the radiosensitivity of esophagus cancer cells by regulating the expressions of proliferation-related protein Ki67 and apoptosis-related proteins Bax and Bcl-2. |
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