| Shi Panying,Lin Wenwen,Zhang Baoguo.Radiosensitizing effects of miR-101 on HeLa cancer cells and underlying mechanism[J].Chinese Journal of Radiological Medicine and Protection,2016,36(12):888-892 |
| Radiosensitizing effects of miR-101 on HeLa cancer cells and underlying mechanism |
| Received:July 11, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.12.002 |
| KeyWords:miR-101 HeLa cells Radiosensitization DNA Repair |
| FundProject:江苏省自然科学基金面上项目（BK20141084） |
| Author Name | Affiliation | E-mail | | Shi Panying | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China | | | Lin Wenwen | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China | | | Zhang Baoguo | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China | bgzhang@suda.edu.cn |
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| Abstract:: |
| Objective To study the effects of microRNA101(miR-101)on radiosensitization of human uterine cervix cancer HeLa cells and underlying mechanism. Methods HeLa cells were divided into three groups including blank control, miRNA negative control and miR-101 transfection group. The cells were irradiated by 160 kVp X-ray generated from a linear accelerator at a dose rate of 1.15 Gy/min. Real-time quantitative PCR (qRT-PCR) was used to detect the expression of miR-101. The clonogenic survival assay was applied to evaluate the effect of miR-101 on radiosensitization of HeLa cells. γ-H2AX immunofluorescence and Western blot assays were performed to observe DNA double-strand breaks and the protein expressions of ATM and DNA-PKcs of HeLa cells, respectively. Results Compared with the negative control group, the expression of miR-101 was significantly increased in the HeLa cells at 48 h after transfection with miR-101 mimic, and the survival of HeLa cells over expression of miR-101 was significantly reduced(t=10.75,P<0.05). The miR-101 had remarkable radiosensitive effect on HeLa cells(F=7.72,P<0.05) with a SERD0 of 1.29. Moreover, over-expression of miR-101 could inhibit the repair of DNA damage induced by irradiation. Compared with the control group, the protein expressions of ATM and DNA-PKcs were significantly decreased in the HeLa cells over expression of miR-101. Conclusions Over-expressions of miR-101 could inhibit cell growth and enhance radiosensitivity of HeLa cells by inhibiting the repair of radiation-induced DNA damage. |
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