| Zhu Chuandong,Wang Lixue,Wang Guoxiang,Ding Jianxun,Xu Hanfeng,Tong Jinlong,Zheng Qin.Synthesis of novel gold nanoparticles and its radiosensitizing effect on HepG2 cells[J].Chinese Journal of Radiological Medicine and Protection,2016,36(12):881-887 |
| Synthesis of novel gold nanoparticles and its radiosensitizing effect on HepG2 cells |
| Received:June 30, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.12.001 |
| KeyWords:Gold nanoparticle Galactose γ-H2AX Radiosensitization Hepatocellular carcinoma cells |
| FundProject:江苏省自然科学基金面上项目（BK20141084） |
| Author Name | Affiliation | E-mail | | Zhu Chuandong | Department of Oncology, The Second Hospital Affiliate to Southeast University, Nanjing 210003, China | | | Wang Lixue | Department of Oncology, The Second Hospital Affiliate to Southeast University, Nanjing 210003, China | | | Wang Guoxiang | Nanjing Zetect Bioscience Incorporated, Nanjing 210061, China | | | Ding Jianxun | Key Laboratory of Polymer Ecomaterials, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 30022, China | | | Xu Hanfeng | Department of Oncology, The Second Hospital Affiliate to Southeast University, Nanjing 210003, China | | | Tong Jinlong | Department of Oncology, The Second Hospital Affiliate to Southeast University, Nanjing 210003, China | | | Zheng Qin | Department of Oncology, The Second Hospital Affiliate to Southeast University, Nanjing 210003, China | njzq83626472@sina.com |
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| Abstract:: |
| Objective To synthesize novel gold nanoparticles of GAL-PEG-GNPs, study its radiation effect on hepatocellular carcinoma cells HepG2 cells in vitro, and investigate the underlying mechanisms. Methods GAL-PEG-GNPs were synthesized and characterized successfully. HepG2 cells were divided into three groups of control, GNPs and GAL-PEG-GNPs. The cytotoxicities of these compounds were tested by the CCK-8 assay and their IC50 values of HepG2 cells were calculated. Cell uptake of nanoparticles was detected by TEM and ICP-MS. The radiosensitization effect of nanoparticles was tested by the colony formation assay. Cell cycle distribution was detected by FCM. The expressions of CAT, SOD, and total GSH were detected with a microplate reader, and the expressions of apoptosis-related proteins were tested by Western blot. Results The GNPs and GAL-PEG-GNPs had absorption peaks at 520 and 530 nm, respectively, and their diameters were (22.6±2.12) and (32.0±1.41) nm detected by ICP-MS. The GAL-PEG-GNPs and GNPs had similar cytotoxicity profiles (P>0.05), while GAL-PEG-GNPs could be more effectively uptaken by HepG2 cells than GNPs. The sensitive enhancement ratio (SER) of GNPs and GAL-PEG-GNPs to HepG2 cells were 1.46和1.95, respectively. The percentage of cells at phase of G2/M in HepG2 population treated with GNP was higher than that of untreated cells (t=14.20, P<0.05). The protein expressions of Cytochrome C, Bax, Caspase-3, and Caspase-9 were up-regulated while Bcl-2 expression was down-regulated in the cells treated with GNPs/radiation or GAL-PEG-GNPs/radiation. The expressions of CAT, SOD and total GSH in the GNP treated groups were significantly decreased compared with the control group(t=12.34, 29.39, 12.85, P<0.05). Conclusions GAL-PEG-GNPs has obvious radiosensitization effect on HepG2 cells, which is related to the induction of cell apoptosis and the generation of free radicals. |
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