| Gao Caixia,Qiao Tiankui,Zhang Bin,Yuan Sujuan.Inhibition of TLR9 expression by siRNA influences radiosensitivity of lung cancer A549 cells[J].Chinese Journal of Radiological Medicine and Protection,2016,36(11):806-810,831 |
| Inhibition of TLR9 expression by siRNA influences radiosensitivity of lung cancer A549 cells |
| Received:May 31, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.11.002 |
| KeyWords:TLR9 expression Lung cancer cells A549 Targeted siRNA Radiosensitivity |
| FundProject:国家自然科学基金（81502664，81172127，81402541）；科技部科研院所技术发展研究专项（2014EG150134）；天津科技支撑计划项目（14ZCZDSY00001） |
| Author Name | Affiliation | E-mail | | Gao Caixia | Department of Oncology, Jinshan Hospital, Fudan University, Shanghai 201508, China | | | Qiao Tiankui | Department of Oncology, Jinshan Hospital, Fudan University, Shanghai 201508, China | qiaotk@163.com | | Zhang Bin | Department of Oncology, Jinshan Hospital, Fudan University, Shanghai 201508, China | | | Yuan Sujuan | Department of Oncology, Jinshan Hospital, Fudan University, Shanghai 201508, China | |
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| Abstract:: |
| Objective To study the influence of TLR9 on the radiosensitivity of radiation-resistance lung cancer A549 cells (R-A549). Methods TLR9 siRNA was transfected to R-A549 cells with liposome and TLR9 expression level was examined by Western blot. After X-ray irradiation with doses from 0 to 8 Gy, colony formation assay was performed and cell survival curve was fitted with the multi-target single-hitting model and D0, Dq, and N value of the survival of A549, R-A549, control siRNA transfected and TLR9 siRNA transfected cells was calculated. Cell proliferation ability and cell cycle distributions of these four groups were also determined. Results TLR9 siRNA successfully suppressed TLR9 expression in R-A549 cells and reduced the colony formation after irradiation with a sensitization enhancement ratio (SERD0) of 1.37 to R-A549. SERD0 of A549 to R-A549 was 1.09. After irradiation, compared to R-A549, cells in G2/M phase of TLR9 siRNA transfected population increased significantly (t=4.323, P<0.05) but G1/G0 phase decreased significantly (t=7.616, P<0.05). Conclusions TLR9 siRNA increased the radiosensitivity of R-A549. Radiation induced G2/M phase arrest and G1/G0 phase decrease could suppress potential lethal damage repair and results in the enhancement of radiation sensitivity. |
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