| Tong Peng,Tu Xumin,Wang Chunyan,Li Ning,Tian Mei,Gou Qiao,Shao Shuai,Qu Gonglin,Li Chen,Qi Xuesong.Effects of cerium oxide nanoparticles on mouse immune system injury induced by γ-ray irradiation[J].Chinese Journal of Radiological Medicine and Protection,2016,36(9):655-659 |
| Effects of cerium oxide nanoparticles on mouse immune system injury induced by γ-ray irradiation |
| Received:May 20, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.09.003 |
| KeyWords:Cerium oxide nanoparticles Apoptosis Cytokine Activity of natural killer cells Immune function |
| FundProject:首都临床特色应用研究与成果推广科研项目（2015—2018年，Z151100004015171） |
| Author Name | Affiliation | E-mail | | Tong Peng | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tu Xumin | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Wang Chunyan | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Li Ning | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Tian Mei | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Gou Qiao | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Shao Shuai | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Qu Gonglin | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Li Chen | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | | | Qi Xuesong | Key Laboratory of Radiological Protection and Nuclear Emergency, China CDC, National Institute for Radiological Protection, Chinese Center for Disease Control and Prevention, Beijing 100088, China | cedar121@sina.com |
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| Abstract:: |
| Objective To study the effects of cerium oxide nanoparticle on immune system injury induced by γ-ray irradiation. Methods BALB/c mice were randomly divided into 6 groups (5 mice each group) including normal control, irradiation group, positive control group, 100 mg/kg CeO2 nanoparticle group, 300 mg/kg CeO2 nanoparticle group and 900 mg/kg CeO2 nanoparticle group. All mice were irradiated in whole body by 3.5 Gy 60Co γ-rays except the normal control group. They were treated with drugs by intragastrical administration from 2 weeks before exposure till death. The apoptosis rate of thymus lymphocytes and the positive rate of IL-2 and IFN-γ in peripheral blood were examined by flow cytometry. The activity of natural killer cells was examined by MTT. Results Compared with the irradiation control group, at 3 d after exposure, the death rates of thymus lymphocytes of the CeO2 nanoparticle groups were decreased, especially in the medium dose group (F=4.50, P<0.05). The positive rate of IFN-γ of the medium dose group was also increased (t=2.26, P<0.05). At 8 d after irradiation, the death rate of the high dose group was declined significantly (F=4.07, P<0.05), the early apoptosis rate of low dose group were declined sharply (F=3.47, P<0.05), and the positive rate of IFN-γ of the medium dose group was increased significantly (t=2.47, P<0.05). The positive rates of IL-2 of CeO2 nanoparticle groups were all decreased, but had no significant differences (P>0.05). The activity of natural killer cells of the CeO2 nanoparticle groups and positive control group were higher than that of control (t=3.40, 5.40, 3.40, 5.20, P<0.05). Conclusions CeO2 nanoparticle can reduce the death rate and the apoptosis rate of the thymus lymphocytes in the γ-ray irradiated mice by increasing the expressions of IL-2 and IFN-γ, the activity of natural killer cells and the mice immunity. CeO2 nanoparticles may have protective effects against radiation injury. |
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