| Zhang Ruifeng,Dang Xuhong,Yuan Yayi,et al.Proteomics in human lymphoblastoid cells after 12C6+ ion irradiation[J].Chinese Journal of Radiological Medicine and Protection,2016,36(9):648-654 |
| Proteomics in human lymphoblastoid cells after 12C6+ ion irradiation |
| Received:January 28, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.09.002 |
| KeyWords:12C6+ ion Human lymphoblastoid cells Proteomics Isobaric tags for relative and absolute quantitation (iTRAQ) |
| FundProject: |
| Author Name | Affiliation | E-mail | | Zhang Ruifeng | China Institute for Radiation Protection, Taiyuan 030006, China | | | Dang Xuhong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Yuan Yayi | China Institute for Radiation Protection, Taiyuan 030006, China | | | Dong Juancong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Zhongxin | China Institute for Radiation Protection, Taiyuan 030006, China | | | Ren Yue | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zuo Yahui | China Institute for Radiation Protection, Taiyuan 030006, China | | | Duan Zhikai | China Institute for Radiation Protection, Taiyuan 030006, China | duanzhikai@cirp.org.cn |
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| Abstract:: |
| Objective To investigate the changes of proteomics in human lymphoblastoid cells after 12C6+ ion irradiation. Methods Human lymphoblastoid cells were irradiated with 12C6+ ion of 0, 0.1, 0.5 and 2.0 Gy at the dose rates of 0.3-0.5 Gy/min. Isobaric tags for relative and absolute quantitation (iTRAQ) was used to analyze the different protein expression in the irradiated cells. Gene ontology(GO), KEGG pathway and protein-protein interaction network analyses were performed. ResultsIn total identified 5 158 kinds of proteins, there were 91, 191, and 68 proteins being differentially expressed in the cells irradiated with 0.1, 0.5 and 2.0 Gy of 12C6+ ions, respectively, where 11 kinds of proteins were commonly expressed (7 up-regulated and 4 down-regulated) in these three groups compared with control. The analysis with bio-information softwares supposed that these differentially expressed proteins may play important roles in the metabolic process and regulation, protein binding and catalytic activity where fibronectin 1 (FN1) and heat shock protein 1B (HSPA1B) might be the key nodes. Conclusions Irradiation of 12C6+ ions can induce differentially expressed proteins in human lymphoblastoid cells. Analysis on functional roles of SZT2, FN1, and HSPA1B may provide new insight into further mechanistic investigations underlying the molecular biomarkers of 12C6+ ion irradiation. |
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