| Ming Hui,Gao Jingmei,Fang Lei,Li Chengxia,Ji Yanhui,Shen Yiming,Hu Yiming,Chang Jin,Li Wei,Tan Jian.Evaluation of SPECT/CT imaging and internal therapeutic effectiveness of 131I-RGD-BSA-PCL in the lung cancer mouse model[J].Chinese Journal of Radiological Medicine and Protection,2016,36(9):641-647 |
| Evaluation of SPECT/CT imaging and internal therapeutic effectiveness of 131I-RGD-BSA-PCL in the lung cancer mouse model |
| Received:March 31, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.09.001 |
| KeyWords:Radioiodine Arg-Gly-Asp (RGD) Nano-liposome NSCLC |
| FundProject:国家自然科学基金（81301244） |
| Author Name | Affiliation | E-mail | | Ming Hui | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Gao Jingmei | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Fang Lei | Tianjin University, School of Life Sciences, Tianjin 300072, China | | | Li Chengxia | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Ji Yanhui | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Shen Yiming | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Hu Yiming | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | | | Chang Jin | Tianjin University, School of Life Sciences, Tianjin 300072, China | | | Li Wei | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | weiwei_tianjin@foxmail.com | | Tan Jian | Department of Nuclear Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China | |
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| Abstract:: |
| Objective To investigate the SPECT/CT imaging of non-small-cell lung carcinoma (NSCLC) mice models and the internal irradiation biological effects and therapeutic effectiveness of nanoliposome 131I-RGD-BSA-PCL. Methods RGD-BSA-PCL and BSA-PCL were constructed. The target binding and cellular uptake in H460 cell line were observed by fluorescence confocal microscopy in vitro. The nanoliposome with 131I were labeled using the Chloramine-T method. Apoptosis analyses was performed using flow cytometry. By construcing tumor xenografts, the biological distribution, change of tumor volume and the SPECT/CT imaging were discussed. Results Confocal microscopy revealed significant uptake of RGD-BSA-PCL or BSA-PCL in NCI-H460 cell after nanolipsome incubated for 1 and 8 h. The early apoptosis rates of Na131I, 131I-BSA-PCL and 131I-RGD-BSA-PCL were (33.3±12.5)%, (68.4±8.0)% and (70.5±12.2)%, respectively. The tumor uptake levels of 131I-BSA-PCL were higher than that of 131I-BSA-PCL (t=9.53, 5.03, P<0.01). There was a significant difference of tumor volume between the treatment group and the control group, and the tumor volume inhibition was most obvious in the 131I-RGD-BSA-PCL group on day 23 post-treatment (t=126.44, P<0.01). SPECT/CT tomography showed that 131I-RGD-BSA-PCL and 131I-BSA-PCL groups had obvious accumulation in the tumor on day 21 post-treatment, and intensity of radiation signal of 131I-RGD-BSA-PCL group was stronger than that of 131I-BSA-PCL group. Conclusions Radionuclide therapy using 131I-RGD-BSA-PCL, which showed excellent targeted cell killing and suppressed tumor growth, exhibited favorable intracellular retention of 131I. |
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