| Dang Xuhong,Zhang Ruifeng,Liu Hongyan,Dong Juancong,Yuan Yayi,Wang Chao,Zhang Zhongxin,Duan Zhikai,Zuo Yahui.Analysis of gene transcriptional profile in human lymphoblastoid cells irradiated by heavy ions of 56Fe17+ and 12C6+[J].Chinese Journal of Radiological Medicine and Protection,2016,36(8):577-582 |
| Analysis of gene transcriptional profile in human lymphoblastoid cells irradiated by heavy ions of 56Fe17+ and 12C6+ |
| Received:February 04, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.08.005 |
| KeyWords:56Fe17+ions 12C6+ions Lymphoblastoid cells Genechip Differential expression genes[WT][HK |
| FundProject: |
| Author Name | Affiliation | E-mail | | Dang Xuhong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Ruifeng | China Institute for Radiation Protection, Taiyuan 030006, China | | | Liu Hongyan | China Institute for Radiation Protection, Taiyuan 030006, China | | | Dong Juancong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Yuan Yayi | China Institute for Radiation Protection, Taiyuan 030006, China | | | Wang Chao | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Zhongxin | China Institute for Radiation Protection, Taiyuan 030006, China | | | Duan Zhikai | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zuo Yahui | China Institute for Radiation Protection, Taiyuan 030006, China | yahuiz@163.com |
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| Abstract:: |
| Objective To investigate the changes of gene transcriptional profile in human lymphocyte irradiated by heavy ion.Methods The agilent human gene expression microaray was used to detect and analysis the transcriptional profiles of human lymphoblastoid cells exposed to 0.1, 0.5 and 2 Gy of heavy ions of 56Fe17+ and 12C6+. The differential expressed genes were analyzed using the GO (gene ontology) database, and the gene pathway was analyzed using the KEGG database. Results Heavy ion irradiation affected the gene expressions in human lymphoblastoid cells. The transcription levels for101, 199, and 299 genes were changed in the lymphoblastoid cells exposed to 0.1, 0.5 and 2 Gy of 56Fe17+ion and 12C6+ion, respectively. A total of 14 differential genes such as GPSM1, TSPYL5, WFDC2, LAD1 were commonly altered in the irradiated cells. The GO and KEGG Pathway analyses indicated that the altered genes in the cells irradiated with 0.1 Gy and 0.5 Gy of 56Fe17+and 12C6+ associated with cell adhesion, extracellular matrix organization, and cytokine-cytokine receptor interaction. The altered genes in 2 Gy groups associated with cell adhesion, regulation of Rho protein signal transduction, and p53 pathway. Conclusions Heavy ion irradiation could alter the gene transcriptional profile in human lymphoblastoid cells in a dose-dependent manner. |
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