| Yuan Yayi,Zhang Ruifeng,Dong Juancong,et al.Effects of 56Fe17+, 12C6+ heavy-ion and 60Co γ-ray irradiation in chromosome aberrations, cell cycle and apoptosis of lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,2016,36(8):572-576 |
| Effects of 56Fe17+, 12C6+ heavy-ion and 60Co γ-ray irradiation in chromosome aberrations, cell cycle and apoptosis of lymphocytes |
| Received:February 03, 2016 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.08.004 |
| KeyWords:56Fe17+ion beam 12C6+ion beam 60Co γ-rays Chromosome aberrations Cell apoptosis |
| FundProject: |
| Author Name | Affiliation | E-mail | | Yuan Yayi | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Ruifeng | China Institute for Radiation Protection, Taiyuan 030006, China | | | Dong Juancong | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zhang Zhongxin | China Institute for Radiation Protection, Taiyuan 030006, China | | | Liu Hongyan | China Institute for Radiation Protection, Taiyuan 030006, China | | | Wang Chao | China Institute for Radiation Protection, Taiyuan 030006, China | | | Duan Zhikai | China Institute for Radiation Protection, Taiyuan 030006, China | | | Zuo Yahui | China Institute for Radiation Protection, Taiyuan 030006, China | yahuiz@163.com |
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| Abstract:: |
| Objective To compare the radiation effects of 56Fe17+, 12C6+ion beams and 60Co γ-rays on chromosome aberrations, cell cycle arrest and apoptosis induction in lymphocytes.Methods Lymphocytes Peng-EBV cells were exposed to 56Fe17+, 12C6+ions and 60Co γ-rays with different dose (0, 0.5, 2.0 Gy) at dose rates of 0.26-0.55, 0.30-0.50 and 0.75 Gy/min, respectively. Chromosome aberration and "d+r" distortion rate was assayed, and cell apoptosis and cell cycle were analyzed by flow cytometry. Results The percentage of cells with chromosome aberration increased significantly after exposure to12C6+, 56Fe17+ion beams and 60Co γ-rays(χ2=12.08-322.9, P<0.05), and the rate of "d+r" distortion was significantly increased as well(χ2=4.06-205.37, P<0.05). The chromosome aberration rate and "d+r" distortion rateinduced by 56Fe17+and 12C6+ ion beams were higher than that of γ-rays, especially 12C6+ irradiation. The G\-2 phase arrest(t=-80.9-0.17,P<0.05)and cell apoptosis(t=-22.65-0.87,P<0.05)were significantly induced by each irradiation in an order of 12C6+, γ-rays and 56Fe17+ ions from high to low. Conclusions Associated with the LET values, 12C6+, 56Fe17+ ionbeams and 60Co γ-rays could induce chromosome aberrations, G2 phase arrest and apoptosis in human lymphocytes, where 12C6+ ion beam irradiation was much more effective. |
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