| Kang Hong,Yang Hui,Ke Shaobo,Zhou Fuxiang,Zhou Yunfeng.Radiation induced expression of HMGB1 in the cells with different radiosensitivity[J].Chinese Journal of Radiological Medicine and Protection,2016,36(7):486-490 |
| Radiation induced expression of HMGB1 in the cells with different radiosensitivity |
| Received:December 11, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.07.002 |
| KeyWords:High mobility group box 1 Radioresistance Radiosensitive Ionizing radiation |
| FundProject:国家自然科学基金（81472799） |
| Author Name | Affiliation | E-mail | | Kang Hong | Department of Radiation Oncology and Medical Oncology, Zhongnan Hospital Affiliated to Wuhan University Hubei Key Laboratory of Tumor Biological Behavior, Wuhan 430071, China | | | Yang Hui | Department of Radiation Oncology and Medical Oncology, Zhongnan Hospital Affiliated to Wuhan University Hubei Key Laboratory of Tumor Biological Behavior, Wuhan 430071, China | | | Ke Shaobo | Department of Radiation Oncology and Medical Oncology, Zhongnan Hospital Affiliated to Wuhan University Hubei Key Laboratory of Tumor Biological Behavior, Wuhan 430071, China | | | Zhou Fuxiang | Department of Radiation Oncology and Medical Oncology, Zhongnan Hospital Affiliated to Wuhan University Hubei Key Laboratory of Tumor Biological Behavior, Wuhan 430071, China | | | Zhou Yunfeng | Department of Radiation Oncology and Medical Oncology, Zhongnan Hospital Affiliated to Wuhan University Hubei Key Laboratory of Tumor Biological Behavior, Wuhan 430071, China | yfzhouwhu@163.com |
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| Abstract:: |
| Objective To investigate the effect of ionizing radiation (IR) on the expressions of HMGB1 in the radiation-sensitive and radiation-resistant human cervical cancer cells and to analysis the role of HMGB1 in the regulation of radiosensitivity. Methods Human cervical cancer cells HeLa and its radioresistant strain HeLaR cells were irradiated with different doses of X-rays. The cells were collected at different time points after irradiation. The expressions of protein and mRNA of HMGB1 were detected by Western blot and real-time quantitative PCR. Results At the protein level, the expression of HMGB1 in HeLaR cells was significantly reduced at 6-36 h after 2, 5 and 10 Gy X-ray irradiation (t=3.574-9.754, P < 0.05), and then it was recovered to the control level at 48 h after IR. On the contrary, the expression of HMGB1 in HeLa cells was significantly increased at 6, 12, 48 h after 2 Gy IR (t=3.945-4.864, P < 0.05), at 6, 36, 48 h after 5 Gy IR (t=-2.875-3.295, P < 0.05), and at 36, 48 h after 10 Gy IR (t=-4.480, -4.517, P < 0.05). At mRNA level, the trend of HMGB1 expression alteration was consistent with that of protein expression. Conclusions The changes of HMGB1 expression can be differently induced by X-rays in the human cervical cancer radiation-sensitivity cells and radiation-resistant cells. HMGB1 may be involved in the radioresistance of human cervical cancer. |
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