| Liu Xiguang,Zhang Hongjun,Song Tingting,Li Jingdong.The effect of apoptosis-2 ligand on irradiation-induced apoptosis in lung adenocarcinima H1975 cells resistant to EGFR-TKI[J].Chinese Journal of Radiological Medicine and Protection,2016,36(6):424-429 |
| The effect of apoptosis-2 ligand on irradiation-induced apoptosis in lung adenocarcinima H1975 cells resistant to EGFR-TKI |
| Received:September 03, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.06.005 |
| KeyWords:Apoptosis-2 ligand Apoptosis Irradiation H1975 cell-line |
| FundProject: |
| Author Name | Affiliation | | Liu Xiguang | Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao 266003, China | | Zhang Hongjun | Department of Oncology, Affiliated Hospital of Qingdao University, Qingdao 266003, China | | Song Tingting | Department of Oncology, Qingdao Central Hospital, Qingdao 266042, China | | Li Jingdong | Department of Oncology, Linyi People's Hospital, Linyi 276003, China |
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| Abstract:: |
| Objective To investigate whether the apoptosis-2 ligand (Apo-2L), known as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), could enhance irradiation-induced apoptosis in lung adenocarcinima H1975 cells that are resistant to the epithelial growth factor receptor (EGFR)-TKI. Methods Adenocarcinima H1975 cells were randomly divided into four groups:the control group, Apo-2L group, irradiation group, and both Apo-2L and irradiation group. H1975 cells were pretreated with Apo-2L under different concentrations of 200 and 228 ng/ml at 24 h before irradiation with doses of 1,1.5,2,2.5,3,3.5 and 4 Gy. The apoptosis rates of all groups were analyzed by flow cytometry 24 h post-irradiation. The inhibition rates of cell proliferation were measured by the MTT assay. Results MTT assay showed that the Apo-2L treatment significantly inhibited cell proliferation(χ2=136.17,P<0.05). The apoptosis rates of the four groups were different significantly, and the apoptosis rate of radiation combined with drug group was significantly higher than the other three groups(χ2=78.02,P<0.05). Conclusions The Apo-2L could not only inhibit the proliferation but also promote radiation-induced apoptosis of adenocarcinoma H1975 cells. |
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