| Lin Wenwen,Shi Panying,Zhang Baoguo.The feasibility study of radiation absorbed dose measurement using oligonucleotide dually labeled with Alexa Fluor 488 and BHQ1 probe[J].Chinese Journal of Radiological Medicine and Protection,2016,36(6):419-423 |
| The feasibility study of radiation absorbed dose measurement using oligonucleotide dually labeled with Alexa Fluor 488 and BHQ1 probe |
| Received:December 10, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.06.004 |
| KeyWords:Fluorescent probe Alexa Fluor 488 BHQ1 |
| FundProject: |
| Author Name | Affiliation | E-mail | | Lin Wenwen | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China | | | Shi Panying | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China | | | Zhang Baoguo | School of Radiation Medicine and Protection, Medical College of Soochow University, Collaborative Innovation Center of Radiological Medicine of Jiangsu Higher Education Institutions, Suzhou 215123, China | bgzhang@suda.edu.cn |
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| Abstract:: |
| Objective To study the feasibility of measuring radiation absorbed dose with the fluorescent probe Alexa Fluor 488-DNA-BHQ1. Methods An oligonucleotide dually labeled at its 5'-and 3'-end with fluorescent molecular Alexa Fluor 488 and specific fluorescence inhibitors BHQ1 was prepared. The Alexa Fluor 488-DNA-BHQ1 aqueous solution was exposed with X-ray and its fluorescence intensity was measured. Results When the concentration of Alexa Fluor 488-DNA-BHQ1 was between 0.5 and 1 μmol/L, the fluorescence intensity of its aqueous solution had excellent linear dose response from 0.1 to 30 Gy (R2=0.99) and it was stably maintained after 40-80 min of irradiation especially at 4℃. Conclusions In the dose range of 0.1-30 Gy, the Alexa Fluor 488-DNA-BHQ1 fluorescent probe can be used to measure radiation absorbed dose. |
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