| Lin Yanyun,Wang Feng,Wang Haibo,Jiang Dapeng,Gao Peng,Ren Zhenghua,Wu Tao,Lang Haiyang,Zeng Lihua,Guo Guozhen,Ding Guirong.Radioprotective effects of nitroxide compound NHCOCH3-TEMPO in BALB/c mice[J].Chinese Journal of Radiological Medicine and Protection,2016,36(6):401-405 |
| Radioprotective effects of nitroxide compound NHCOCH3-TEMPO in BALB/c mice |
| Received:November 09, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.06.001 |
| KeyWords:Nitroxide compound BALB/c mice γ-rays Radiation protection |
| FundProject:国家科技支撑计划(2013BAK03B05);国家973计划(2011CB503704);国家自然科学基金(81272490,31470828);军队指令性项目(13BJYZ29) |
| Author Name | Affiliation | E-mail | | Lin Yanyun | Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | | | Wang Feng | CT Department, Shanxi Province Corps Hospital, Chinese People's Armed Police Forces, Taiyuan 030006, China | | | Wang Haibo | Department of Chemistry, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | | | Jiang Dapeng | Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | | | Gao Peng | Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | | | Ren Zhenghua | Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | | | Wu Tao | Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | | | Lang Haiyang | Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | | | Zeng Lihua | Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | | | Guo Guozhen | Department of Radiation Medicine, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | | | Ding Guirong | Department of Radiation Biology, Faculty of Preventive Medicine, The Fourth Military Medical University, Xi'an 710032, China | dingzhao@fmmu.edu.cn |
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| Abstract:: |
| Objective To investigate the protective effects of nitroxide compound NHCOCH3-TEMPO against γ-ray irradiation-induced injury in male BALB/c mice. Methods 120 BALB/c male mice were randomly divided into two super group, each super group then were randomly divided into 6 groups:control group, normal saline(NS)+γ group, low-dose TEMPO+γ group, middle-dose TEMPO+γ group, high-dose TEMPO+γ group and the positive control (WR-2721)+γ group. BALB/c mice were intraperitoneally injected with nitroxide compound at 0.5 h before whole body 60Co γ-ray irradiation. The drug dosages were as follows:WR-2721 group 200 mg/kg, low-dose TEMPO group 100 mg/kg, middle-dose TEMPO group 200 mg/kg, high-dose TEMPO group 400 mg/kg. The first super group of the mice was given 7 Gy γ-ray whole-body irradiation, and then 30 d survival rate was observed. The second super group of the mice was given 4 Gy γ-ray whole-body irradiation. The number of nucleated cells and the content of DNA in bone marrow, as well as the blood routine were detected. Results At 8th and 15th day after 4 Gy γ-ray irradiation, the number of bone marrow nucleated cells, the content of DNA significantly increased in TEMPO+γ-ray groups compared with NS+γ-ray group(t=2.53-6.13,P<0.05). The number of white blood cells significantly increased in middle dose TEMPO+γ-ray groups compared with NS+γ-ray group(t=4.34,P<0.05), while there was no significant difference in the number of red blood cells between the TEMPO+γ-ray group and the NS+γ-ray group. After 7 Gy γ-ray irradiation, the survival rate of mice on the 30th day significantly increased in low-dose TEMPO+γ-ray group compared with NS+γ-ray group(χ2=5.934,P<0.05). Conclusions The nitroxide compound NHCOCH3-TEMPO could provide radio protective effects in BALB/c mice. |
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