| Zuo Xiaoyong,Song Shujun,Si Shaoyan,et al.Protective effect of vitamin D on the immune function of mice in response to radiation[J].Chinese Journal of Radiological Medicine and Protection,2016,36(4):268-271 |
| Protective effect of vitamin D on the immune function of mice in response to radiation |
| Received:December 23, 2015 |
| DOI：10.3760/cma.j.issn.0254-5098.2016.04.007 |
| KeyWords:Vitamin D Radiation Immune function |
| FundProject: |
| Author Name | Affiliation | E-mail | | Zuo Xiaoyong | 306th Clinical College of PLA, Anhui Medical University, Beijing 100101, China | | | Song Shujun | Center for Special Medicine and Experimental Research, 306th Hospital of PLA, Beijing 100101, China | | | Si Shaoyan | Center for Special Medicine and Experimental Research, 306th Hospital of PLA, Beijing 100101, China | | | Wang Ying | Center for Special Medicine and Experimental Research, 306th Hospital of PLA, Beijing 100101, China | | | Shan Gaixian | Department of Radiation Oncology, 306th Hospital of PLA, Beijing 100101, China | | | Ren Ye | Department of Radiation Oncology, 306th Hospital of PLA, Beijing 100101, China | | | Dai Zhuojie | Department of Radiation Oncology, 306th Hospital of PLA, Beijing 100101, China | | | Wang Zongye | 306th Clinical College of PLA, Anhui Medical University, Beijing 100101, China | wangzye@163.com |
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| Abstract:: |
| Objective To investigate the effect of vitamin D on the immune function of mice irradiated with different doses of X-rays. Methods The mice were randomly divided into five groups: controls, 2 and 5 Gy irradiated groups, 2 and 5 Gy X-rays combined with vitamin D groups. Vitamin D (6 IU/g) was intraperitoneally injected into the mice after irradiation for 7 days. On day 8, all mice were killed, and body, thymus and spleen of each mouse were weighed. The thymus and spleen indexes, and proliferation of spleen T lymphocytes were assessed. The level of IL-2 in the supernatant of spleen cells was measured by ELISA. Results Compared with control group, proliferation of spleen T lymphocytes and the level of IL-2 in the supernatant significantly decreased in 2 and 5 Gy groups (F=36.20,7.13, P<0.05). Compared with 2 Gy group, the thymus index, spleen index and proliferation of spleen T lymphocytes were significantly increased in the 2 Gy plus vitamin D group (t=-2.54,-2.24,-2.84, P<0.05). Compared to the 5 Gy group, the thymus index and the level of IL-2 in the supernatant significantly increased in the 5 Gy plus vitamin D group (t=-5.02,-2.64, P<0.05). Conclusions Vitamin D could restore the immune function of mice after radiation, while the restore capacity was decreased along with the increase of radiation dose. |
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